Overview

  • Product name
  • Description
    Rabbit polyclonal to KMT6 / EZH2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ChIP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human KMT6/ EZH2 (internal sequence). The exact sequence is proprietary.
    Database link: Q15910

  • Positive control
    • WB: NCI-H1299, HCT 116 and MCF7 whole cell lysates. ICC/IF: HeLa cells. ChIP: HeLa chromatin extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 85 kDa.
ChIP Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
  • Tissue specificity
    Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • Developmental stage
    Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
  • Post-translational
    modifications
    Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Enhancer of zeste 2 antibody
    • enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
    • Enhancer of zeste homolog 2 (Drosophila) antibody
    • Enhancer of zeste homolog 2 antibody
    • Enhancer of zeste, Drosophila, homolog 2 antibody
    • ENX 1 antibody
    • Enx 1h antibody
    • ENX-1 antibody
    • ENX1 antibody
    • Enx1h antibody
    • EZH 2 antibody
    • EZH1 antibody
    • EZH2 antibody
    • EZH2_HUMAN antibody
    • EZH2b antibody
    • Histone-lysine N-methyltransferase EZH2 antibody
    • KMT 6 antibody
    • KMT6 antibody
    • KMT6A antibody
    • Lysine N-methyltransferase 6 antibody
    • MGC9169 antibody
    • WVS antibody
    • WVS2 antibody
    see all

Images

  • All lanes : Anti-KMT6 / EZH2 antibody (ab228697) at 1/1000 dilution

    Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 85 kDa



    7.5% SDS-PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KMT6 / EZH2 (green) using ab228697 at 1/200 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ab228697. The precipitated DNA was detected by PCR with primer set targeting to CCND2.

References

This product has been referenced in:
  • Zheng N  et al. Rottlerin inhibits cell growth and invasion via down-regulation of EZH2 in prostate cancer. Cell Cycle 17:2460-2473 (2018). Read more (PubMed: 30394832) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
ChIP
Sample
Xenopus tropicalis Tissue lysate - whole (embryos)
Negative control
Ezh2 unbound region
Specification
embryos
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 45 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
some known Ezh2 bound regions

Abcam user community

Verified customer

Submitted Oct 17 2018

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