Application
ChIP
Sample
Xenopus tropicalis Tissue lysate - whole (embryos)
Negative control
Ezh2 unbound region
Specification
embryos
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 45 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Duration of cross-linking step: 45 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
some known Ezh2 bound regions
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Additional data
Abcam response
Abcam had not tested the antibody in Xenopus and thus can't say how it should have performed. This individual received a credit for testing the antibody in Xenopus as part of our AbTrial program.
Abcam user community
Verified customer
Submitted Oct 17 2018