Key features and details
- Rabbit polyclonal to KMT6 / EZH2 - ChIP Grade
- Suitable for: ChIP, WB, ChIP-sequencing
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KMT6 / EZH2 antibody - ChIP Grade
See all KMT6 / EZH2 primary antibodies
DescriptionRabbit polyclonal to KMT6 / EZH2 - ChIP Grade
Tested applicationsSuitable for: ChIP, WB, ChIP-sequencingmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cynomolgus monkey
Recombinant fragment corresponding to Mouse KMT6/ EZH2 aa 1-343.
MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKTMFSSNRQKIL ERTETLNQEWKQRRIQPVHIMTSVSSLRGTRECSVTSDLDFPAQVIPLKT LNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELI KNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPDEREEKQ KDLEDNRDDKETCPPRKFPADKIFEAISSMFPDKGTAEELKEKYKELTEQ QLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFH ATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERI
Database link: Q61188
- Chromatin prepared from K562 cells; HeLa nuclear extract.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab195409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.
Use 2 μg/ChIP for chromatin prepared from 4x106 cells.
|WB||1/1000. Predicted molecular weight: 85 kDa.|
|ChIP-sequencing||Use at an assay dependent concentration.
Use 2 μg/ChIP for chromatin prepared from 4x106 cells
FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
Tissue specificityExpressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain.
Developmental stageExpression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
modificationsPhosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
- Information by UniProt
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
ChIP results obtained with ab195409 directed against KMT6 / EZH2.
ChIP assays were performed using K562 cells, ab195409 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIPseq results obtained with ab195409 directed against KMT6 / EZH2.
ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of ab195409. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure C and D).
Anti-KMT6 / EZH2 antibody - ChIP Grade (ab195409) at 1/1000 dilution + HeLa nuclear extract at 40 µg
Predicted band size: 85 kDa
The antiody was diluted in TBS-Tween containing 5% skimmed milk.
ab195409 has been referenced in 5 publications.
- Xu W et al. Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation. Cancer Cell Int 20:116 (2020). PubMed: 32308561
- He W et al. Downregulation of long noncoding RNA FENDRR predicts poor prognosis in renal cell carcinoma. Oncol Lett 17:103-112 (2019). PubMed: 30655744
- Qiu Z et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
- Garland W et al. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. Cell Rep 29:1800-1811.e6 (2019). PubMed: 31722198
- Rotolo A et al. Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting. Cancer Cell 34:596-610.e11 (2018). PubMed: 30300581