ChIP abreview for Anti-KMT6 / EZH2 antibody - ChIP Grade

Inconclusive
Abreviews
Application
ChIP
Sample
Xenopus tropicalis Tissue lysate - whole (embryos)
Negative control
il22 promoter (Ezh2 unbound region by ab3748)
Specification
embryos
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 45 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
gata4 promoter (Ezh2 bound region by ab3748)

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA
Dilution
1/100

Additional data

Additional Notes
45min fixation of Xenopus tropicalis embryos works for ChIPs with most ChIp grade antibodies.
Abcam response
Thank you for having tested ab195409 in Xenopus tropicalis, an untested species. The alignment of the immunogen with the target protein sequence is 89%. This percentage suggests there could be some cross-reactivity. We are sorry that this is not sufficient to make ab195409 works in this experiment. We welcome any further feedback in this species with our AbTrial program (www.abcam.com/abtrial).

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Submitted Oct 17 2018

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