Application
Western blot
Sample
Human Cell lysate - whole cell (Smooth Muscle Cells)
Gel Running Conditions
Non-reduced Denaturing (1-15%)
Loading amount
15 µg
Specification
Smooth Muscle Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: Milk
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 90 kDa
Additional data
Additional Notes
The samples showed in the picture correspond to human aortic Smooth muscle cells without any treatment. Cultured during 48 hours.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Nov 26 2021