Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9307] to KMT6 / EZH2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Product nameAnti-KMT6 / EZH2 antibody [EPR9307]
See all KMT6 / EZH2 primary antibodies
DescriptionRabbit monoclonal [EPR9307] to KMT6 / EZH2
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Recombinant fragment within Human KMT6/ EZH2 aa 150-300. The exact sequence is proprietary.
Database link: Q15910
- Recombinant Human KMT6 / EZH2 protein (ab132934) can be used as a positive control in WB. MCF7, Jurkat and HeLa cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 85 kDa.|
FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
Tissue specificityExpressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain.
Developmental stageExpression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
modificationsPhosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
- Information by UniProt
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: KMT6 / EZH2 knockout HAP1 cell lysate (40 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab150433 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab150433 was shown to recognize KMT6 / EZH2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when KMT6 / EZH2 knockout samples were examined. Wild-type and KMT6 / EZH2 knockout samples were subjected to SDS-PAGE. Ab150433 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4oC. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) (ab216773)
and IRDye® 680 Goat anti-Mouse IgG (H + L) (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa
ab150433 has been referenced in 3 publications.
- Qiu Z et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
- Wang J et al. Evaluation of EZH2 expression, BRAF V600E mutation, and CDKN2A/B deletions in epithelioid glioblastoma and anaplastic pleomorphic xanthoastrocytoma. J Neurooncol 144:137-146 (2019). PubMed: 31214915
- Jin Q et al. MicroRNA-101-3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Exp Ther Med 16:1663-1670 (2018). PubMed: 30186385