Overview

  • Product name

    Anti-KMT6 / EZH2 antibody [EPR9307]
    See all KMT6 / EZH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9307] to KMT6 / EZH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human KMT6/ EZH2 aa 150-300. The exact sequence is proprietary.
    Database link: Q15910

  • Positive control

    • Recombinant Human KMT6 / EZH2 protein (ab132934) can be used as a positive control in WB. MCF7, Jurkat and HeLa cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
    • Tissue specificity

      Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
    • Sequence similarities

      Belongs to the histone-lysine methyltransferase family. EZ subfamily.
      Contains 1 SET domain.
    • Developmental stage

      Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
    • Post-translational
      modifications

      Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Enhancer of zeste 2 antibody
      • enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
      • Enhancer of zeste homolog 2 (Drosophila) antibody
      • Enhancer of zeste homolog 2 antibody
      • Enhancer of zeste, Drosophila, homolog 2 antibody
      • ENX 1 antibody
      • Enx 1h antibody
      • ENX-1 antibody
      • ENX1 antibody
      • Enx1h antibody
      • EZH 2 antibody
      • EZH1 antibody
      • EZH2 antibody
      • EZH2_HUMAN antibody
      • EZH2b antibody
      • Histone-lysine N-methyltransferase EZH2 antibody
      • KMT 6 antibody
      • KMT6 antibody
      • KMT6A antibody
      • Lysine N-methyltransferase 6 antibody
      • MGC9169 antibody
      • WVS antibody
      • WVS2 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: KMT6 / EZH2 knockout HAP1 cell lysate (40 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab150433 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab150433 was shown to recognize KMT6 / EZH2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when KMT6 / EZH2 knockout samples were examined. Wild-type and KMT6 / EZH2 knockout samples were subjected to SDS-PAGE. Ab150433 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4oC. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) (ab216773)
      and IRDye® 680 Goat anti-Mouse IgG (H + L) (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 85 kDa

    References

    This product has been referenced in:

    • Jin Q  et al. MicroRNA-101-3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Exp Ther Med 16:1663-1670 (2018). Read more (PubMed: 30186385) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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