Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-KMT6 / EZH2 antibody [EPR9307] - BSA and Azide free (ab240119)

Overview

  • Product name

    Anti-KMT6 / EZH2 antibody [EPR9307] - BSA and Azide free
    See all KMT6 / EZH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9307] to KMT6 / EZH2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human KMT6/ EZH2.

  • General notes

    ab240119 is the carrier-free version of ab150433 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240119 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
    • Tissue specificity

      Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
    • Sequence similarities

      Belongs to the histone-lysine methyltransferase family. EZ subfamily.
      Contains 1 SET domain.
    • Developmental stage

      Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
    • Post-translational
      modifications

      Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Enhancer of zeste 2 antibody
      • enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
      • Enhancer of zeste homolog 2 (Drosophila) antibody
      • Enhancer of zeste homolog 2 antibody
      • Enhancer of zeste, Drosophila, homolog 2 antibody
      • ENX 1 antibody
      • Enx 1h antibody
      • ENX-1 antibody
      • ENX1 antibody
      • Enx1h antibody
      • EZH 2 antibody
      • EZH1 antibody
      • EZH2 antibody
      • EZH2_HUMAN antibody
      • EZH2b antibody
      • Histone-lysine N-methyltransferase EZH2 antibody
      • KMT 6 antibody
      • KMT6 antibody
      • KMT6A antibody
      • Lysine N-methyltransferase 6 antibody
      • MGC9169 antibody
      • WVS antibody
      • WVS2 antibody
      see all

    References

    ab240119 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240119.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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