Recombinant
RabMAb

Recombinant Anti-KMT6 / EZH2 antibody [SP129] - BSA and Azide free (ab245738)

Overview

  • Product name
    Anti-KMT6 / EZH2 antibody [SP129] - BSA and Azide free
    See all KMT6 / EZH2 primary antibodies
  • Description
    Rabbit monoclonal [SP129] to KMT6 / EZH2 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey
  • Immunogen

    Synthetic peptide within Human KMT6/ EZH2 aa 300-400. The exact sequence is proprietary.
    Database link: Q15910

  • Positive control
    • WB: HeLa whole cell lysate (ab150035). IHC-P: Human prostate cancer tissue. Flow Cyt: HeLa cells.
  • General notes

    ab245738 is a PBS-only buffer format of ab227648. Please refer to ab227648 for recommended dilutions, protocols, and image data.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab245738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.

Primary antibody incubation for 1 hour at room temperature.

 

IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer before commencing with IHC staining protocol.Primary antibody incubation for 30 minutes at room temperature.

 

Flow Cyt Use at an assay dependent concentration.

Primary antibody incubation for 30 minutes at 4 degrees.

 

Target

  • Function
    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
  • Tissue specificity
    Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • Developmental stage
    Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
  • Post-translational
    modifications
    Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Enhancer of zeste 2 antibody
    • enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
    • Enhancer of zeste homolog 2 (Drosophila) antibody
    • Enhancer of zeste homolog 2 antibody
    • Enhancer of zeste, Drosophila, homolog 2 antibody
    • ENX 1 antibody
    • Enx 1h antibody
    • ENX-1 antibody
    • ENX1 antibody
    • Enx1h antibody
    • EZH 2 antibody
    • EZH1 antibody
    • EZH2 antibody
    • EZH2_HUMAN antibody
    • EZH2b antibody
    • Histone-lysine N-methyltransferase EZH2 antibody
    • KMT 6 antibody
    • KMT6 antibody
    • KMT6A antibody
    • Lysine N-methyltransferase 6 antibody
    • MGC9169 antibody
    • WVS antibody
    • WVS2 antibody
    see all

Images

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling KMT6 / EZH2 with ab227648 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227648).

  • Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for KMT6 / EZH2 using ab227648 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227648).

References

ab245738 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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