Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP129] to KMT6 / EZH2 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt, IP
- Reacts with: Mouse, Human
Product nameAnti-KMT6 / EZH2 antibody [SP129] - C-terminal
See all KMT6 / EZH2 primary antibodies
DescriptionRabbit monoclonal [SP129] to KMT6 / EZH2 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Monkey
Synthetic peptide within Human KMT6/ EZH2 aa 300-400. The exact sequence is proprietary.
Database link: Q15910
- WB: HeLa whole cell lysate (ab150035), NIH/3T3 and HEK-293 cell lysates; Mouse brain tissue lysate. IHC-P: Human prostate cancer tissue. Flow Cyt: HeLa cells. IP: HEK-293 whole cell lysate
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab227648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/25. Predicted molecular weight: 85 kDa.
Primary antibody incubation for 1 hour at room temperature.
Perform heat mediated antigen retrieval with EDTA buffer before commencing with IHC staining protocol.
Primary antibody incubation for 30 minutes at room temperature.
Primary antibody incubation for 30 minutes at 4°C.
FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
Tissue specificityExpressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain.
Developmental stageExpression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
modificationsPhosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
- Information by UniProt
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
All lanes : Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648) at 0.316 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 85 kDa
Observed band size: 85 kDa
Blocking/Diluting buffer and concentration: 5% NFDM /TBST
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for KMT6 / EZH2 using ab227648 at 1/100 dilution in immunohistochemical analysis.
ab227648 (purified) at 1/20 dilution (20 µg/ml) immunoprecipitating KMT6 in HEK-293 whole cell lysate.
Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab227648 & HEK-293 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab227648 in HEK-293 whole cell lysate
For western blotting, ab227648 (purified at 1/500 dilution (0.632 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
Blocking and diluting buffer: 5% NFDM /TBST.
Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648) at 1/25 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 85 kDa
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling KMT6 / EZH2 with ab227648 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227648 has been referenced in 1 publication.
- Singla N et al. Multi-institutional evaluation of the prognostic significance of EZH2 expression in high-grade upper tract urothelial carcinoma. Urol Oncol N/A:N/A (2018). PubMed: 29748098