Product nameAnti-KMT6 / EZH2 antibody
See all KMT6 / EZH2 primary antibodies
DescriptionRabbit polyclonal to KMT6 / EZH2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis
- 293T, HeLa, Jurkat, and Mouse TCMK-1 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 85 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||Use at an assay dependent concentration.|
FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
Tissue specificityExpressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain.
Developmental stageExpression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
modificationsPhosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
- Information by UniProt
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
Lane 1 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg
Lanes 2-4 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 cells.lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 3 minutes
Mouse plasmacytoma tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
Human ovarian tissue stained with ab186006 at a diltution of 1:1000 to detect EZH2.
Detection of KMT6 / EZH2 by Western Blot of Immunprecipitate.
Lane 1: ab186006 at 1 µg/ml labeling KMT6 / EZH2 in 293T whole cell lysate immunoprecipitated using ab186006 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 3 minutes.
This product has been referenced in:
- Chen Y et al. Long noncoding RNA FOXC2-AS1 facilitates the proliferation and progression of prostate cancer via targeting miR-1253/EZH2. Gene 686:37-42 (2019). Read more (PubMed: 30389560) »
- Shivram H et al. MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network. Genome Res 29:184-192 (2019). Read more (PubMed: 30651280) »