Overview

  • Product name
    Anti-KMT8/Riz1/Riz2 antibody
  • Description
    Rabbit polyclonal to KMT8/Riz1/Riz2
  • Host species
    Rabbit
  • Specificity
    This antibody recognizes the beta isoform (Riz 2) of PRDM 2 (185 kDa) as well as RIZ 1. It will not detect isoform 4.
  • Tested applications
    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (GST-tag) corresponding to Rat KMT8/Riz1/Riz2 aa 245-573.

  • Positive control
    • IP: Rat B50 brain tumour cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 189 kDa. PubMed: 24423864
IHC-P 1/100 - 1/500.

Target

  • Function
    S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
  • Tissue specificity
    Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
  • Sequence similarities
    Contains 8 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GATA 3 binding protein G3B antibody
    • GATA-3-binding protein G3B antibody
    • HUMHOXY1 antibody
    • Lysine N methyltransferase 8 antibody
    • Lysine N-methyltransferase 8 antibody
    • MTB ZF antibody
    • MTB-ZF antibody
    • MTE binding protein antibody
    • MTE-binding protein antibody
    • PR domain containing 2 with ZNF domain antibody
    • PR domain containing protein 2 antibody
    • PR domain zinc finger protein 2 antibody
    • PR domain-containing protein 2 antibody
    • PRDM2 antibody
    • PRDM2_HUMAN antibody
    • Retinoblastoma protein binding zinc finger protein antibody
    • Retinoblastoma protein interacting zinc- inger protein antibody
    • Retinoblastoma protein-interacting zinc finger protein antibody
    • RIZ antibody
    • RIZ-LSB antibody
    • RIZ1 antibody
    • RIZ2 antibody
    • Zinc finger DNA binding protein antibody
    • Zinc finger protein RIZ antibody
    see all

Images

  • Rat B50 brain tumor cell extracts were immunoprecipitated with RIZ antibody or preimmune (Liu L. et al. J. Biol. Chem. 1997. 272: 2984.).

  • Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis (Jiang G-L. and Huang S. Cancer Res. 2001. 61: 1796.)

References

This product has been referenced in:
  • Cheedipudi S  et al. A fine balance: epigenetic control of cellular quiescence by the tumor suppressor PRDM2/RIZ at a bivalent domain in the cyclin a gene. Nucleic Acids Res 43:6236-56 (2015). IHC-P ; Mouse . Read more (PubMed: 26040698) »
  • Zhang C  et al. RIZ1: a potential tumor suppressor in glioma. BMC Cancer 15:990 (2015). IHC . Read more (PubMed: 26690953) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

We recently spoke on the phone regarding a Histone H3 methylation study. Below I have summarized our conversation and answers to your questions.

For detecting nuclear proteins via western blot we recommend using a RIPA buffer or cell fractionation protocol in preparation of your cell lysate. I attached our protocol book to this email for information regarding specific cell lysate preparation procedures. Below are the fractionation kits that we talked about during your phone call:
Nuclear Extraction Kit (ab113474) https://www.abcam.com/episeeker-nuclear-extraction-kit-ab113474.html
Histone Extraction Kit (ab113476)
https://www.abcam.com/episeeker-histone-extraction-kit-ab113476.html

The Histone Extraction Kit can be used to extract histones from as low as 1 million cells or 1 mg of tissue as stated on the datasheet. These lower limits are good guidelines for the Nuclear Extraction Kit as well.

Since ab1220 (anti-histone H3 di methyl K9) is the only antibody in your provided list that is raised in mouse (the others are raised in rabbit), we discussed substituting the rabbit monoclonal antibody ab32521 (https://www.abcam.com/histone-h3-di-methyl-k9-antibody-y49-ab32521.html) (anti-histone H3 di methyl K9) so you can use the same secondary for each antibody. Since ab32521 is a rabbit monoclonal antibody, it also qualifies for our RabMAB promotion. Details of this promotion are at the end of this email.

A list of our anti-rabbit secondary antibodies is given through the link below, depending on the detection method and conjugation that you prefer:
https://www.abcam.com/search?AppliedFacets.FacetProductType=Secondary+antibodies&AppliedFacets.FacetReactivity=Rabbit&IsFacetsOnly=False&PageNumber=1&Keywords[0]=rabbit&X-Requested-With=XMLHttpRequest

Together these antibodies could be used to detect the presence of histone H3 in your samples as well as the methylation status of Lysine 9. To complete the spectrum, Abcam also sells an anti-histone H3 mono methyl K9 antibody (ab9045, https://www.abcam.com/histone-h3-mono-methyl-k9-antibody-chip-grade-ab9045.html). As we discussed, ab3790 (anti-KMT8/Riz1/Riz2) detects the presence of PR domain zinc finger protein 2, which is a histone methyltransferase that specifically methylates Lysine 9 of histone H3 according to SwissProt (http://www.uniprot.org/uniprot/Q13029), thus explaining its connection to the anti-histone H3 antibodies that detect the methylation status of Lysine 9.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link:
https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

This antibody has been cited in multiple publications (see list below) as KG7.1S serum by the leader in PRDM2 research. The discrepancies between theorical MWs and observed MWs are due to the experimental procedure (IP and WB) that will detect post-translated-modified PRDM2. Therefore the two isoforms are migrating at a higher MW. Liu L et al. JBC 1997 272: 2984-2991 Jiang GL and Huang S. Cancer Res. 2001 61: 1796-1798 Steele-Perkins G. et al. Genes & Dev. 2001 15: 2250-2262

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