AB133997

Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)

Cytokine Array - Human Cytokine 항체 어레이 (Membrane, 42 Targets)

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Human Cytokine Array Kit - Human Cytokine Antibody Array (Membrane, 42 Targets) ab133997 is a membrane based multiplex immunoassay for the simultaneous detection of 42 human cytokines in all sample types.

- More data, less sample: screen multiple cytokines with the same sample
- Global view of cytokine expression
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

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Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Quantification of Human Cytokine Array (membrane antibody array).

Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Quantification of Human Cytokine Array (membrane antibody array).

Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Cytokine array testing image - Abreview.

The antibody array was used to determine the change in expression of cytokines related to cell proliferation.

One million Human breast cancer cells (MCF7) were cultured in a 10mm dish for 48 hours (in 8ml of RPMI-1640 medium) and were then treated with 5 mM Compound X (not disclosed) for 48 hours. The Abcam protocol was followed for the rest of the procedure. 1 mL of supernatant of culture medium was used for sample incubation (4°C overnight).

Rating : 4.5/5

This image is courtesy of an anonymous Abreview.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Human Cytokine Array - Membrane Antibody Array.

Human peripheral blood cells (1x10⁶ cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.

Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control.

주요 정보

Reacts with

Human

타겟

ANG

target

Applications

Multiplex Protein Detection

applications

샘플 타입

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

ab133997 is a cytokine array to be used for the simultaneous detection of 42 Human cytokines. It is suitable for all sample types.

Targets: ENA-78, GCSF, GM-CSF, GRO, GRO-alpha, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1, MCP-2, MCP-3, MCSF, MDC, MIG, MIP-1delta, RANTES, SCF, SDF-1, TARC, TGF-beta1, TNF-alpha, TNF-beta, EGF, IGF-I, Angiogenin, Oncostatin M, Thrombopoietin, VEGF-A, PDGF BB, Leptin

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, cytokine array ab133998, ab133996, ab169817, ab134003, ab134000, ab169804 and ab169805 may also be of interest.

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

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제품 프로토콜

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타겟 정보

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed : 12051708, PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 23843625, PubMed : 2424496, PubMed : 2459697, PubMed : 2730651, PubMed : 27518564, PubMed : 28176817, PubMed : 29100074, PubMed : 29748193, PubMed : 3122207, PubMed : 32510170, PubMed : 38718836, PubMed : 8159680, PubMed : 8570639, PubMed : 8622921, PubMed : 9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs) : specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 27518564, PubMed : 29100074, PubMed : 29748193, PubMed : 32510170, PubMed : 38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed : 3289612, PubMed : 38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed : 31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed : 12051708, PubMed : 15735021, PubMed : 27518564, PubMed : 29100074, PubMed : 8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed : 19354288, PubMed : 4074709, PubMed : 8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed : 27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity : secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed : 32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone : produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).

See full target information ANG

Additional targets

CXCL5,EGF,CSF3,CSF2,CXCL1,CXCL3,CCL1,IFNG,IGF1,IL1A,IL1B,IL10,IL12A,IL13,IL15,IL2,IL3,IL4,IL5,IL6,IL7,CXCL8,LEP,CCL2,CCL8,CCL7,CSF1,CCL22,CXCL9,CCL15,OSM,PDGFB,CCL5,KITLG,CXCL12,CCL17,TGFB1,THPO,TNF,LTA,VEGFA,CXCL2

대체 명칭 보기

RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5, MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, ENA78, SCYB5, CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5, Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1, D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228, SCYA17, TARC, CCL17, C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, MCP2, SCYA10, SCYA8, CCL8, C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2, GRO2, GROB, MIP2A, SCYB2, CXCL2, C-X-C motif chemokine 2, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha, Gro-beta, MIP2-alpha, TGFB, TGFB1, Transforming growth factor beta-1 proprotein, MDC, SCYA22, A-152E5.1, CCL22, C-C motif chemokine 22, CC chemokine STCP-1, MDC(1-69), Macrophage-derived chemokine, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1, SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Pro-epidermal growth factor, EGF, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3, GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, NC30, IL13, Interleukin-13, IL-13, Interleukin-15, IL-15, IL15, Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5, Interleukin-7, IL-7, IL7, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1, OB, OBS, LEP, Leptin, Obese protein, Obesity factor, MCP3, SCYA6, SCYA7, CCL7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3, CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG, MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5, Oncostatin-M, OSM, MGF, SCF, KITLG, Kit ligand, Mast cell growth factor, Stem cell factor, c-Kit ligand, SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF, MGDF, THPO, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

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제품이 사용된 논문 (47)

Recent publications for all applications. Explore the 전체 목록and refine your search

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Biocooperative Regenerative Materials by Harnessing Blood-Clotting and Peptide Self-Assembly.

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제품이 사용된 논문 모두 보기

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Product promise

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Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)

Cytokine Array - Human Cytokine 항체 어레이 (Membrane, 42 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

주요 정보

Reacts with

타겟

Applications

샘플 타입

보완 제품

Reagents, Controls & Accessories

Multiplex Kits

Secondary Antibodies

Assay Kits

Multiplex Kits

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

제품이 사용된 논문 (47)

Product promise