Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets)
Cytokine Array - Mouse Cytokine 항체 어레이 (Membrane, 32 Targets)
Be the first to review this product! 리뷰 제출
|
(4 제품이 사용된 논문 )
- More data, less sample: screen multiple cytokines with the same sample
- Global view of cytokine expression
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)
- Multiplex Protein Detection
Unknown
Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 32 Targets) (AB133994)
The cytokine antibody array was used to detect changes in cytokine/chemokine secretion by cultured mouse eosinophils treated with recombinant protein of interest or with vehicle control. Briefly, mouse primary eosinophils were harvested and cultured in suspension, 300ng/ml recombinant protein X (our protein of interest) or vehicle alone were added to the cells for 24 hours. Then the conditioned medium were harvested by centrifugation and added directly without dilution onto the cytokine antibody array (the array was first blocked according to manufacturer's protocol). Manufacturer's protocol was followed for the subsequent steps and the end result is shown in the following picture. The amount of three candidate cytokine/chemokines were identified to have changed in the treated conditioned medium : GCSF (H1 and H2), Leptin (K3 and K4), and KC (J3 and J4). The changes of Leptin and KC secretion were subsequently confirmed by ELISA in independent experiments.
Abreview rating 4/5 stars. Review from Abcam user community. Verified customers - Yi Chen, Ph.D. Research Fellow, Spiegelman Lab, Dana-Farber Cancer Institute, Department of Cancer Biology, Boston, USA. Submitted 25-Jun-15.
This image is courtesy of an Abreview submitted by Yi Chen, Ph.D.
Reactivity 정보
제품 세부 정보
ab133994 is for simultaneous detection of 32 Mouse Cytokines. Suitable for all sample types.
Targets: 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40/p70, IL-12 p70, IL-13, IL-17, IFN-gamma, KC/CXCL1, Leptin/OB, MCP-1, MCP-5, MIP-1alpha, MIP-2, MIP-3beta, RANTES, SCF, sTNF RI, TARC, TIMP-1, TNF-alpha, Thrombopoietin, VEGF-A
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
제품 구성
특성 및 보관 정보
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
타겟 정보
Additional targets
대체 명칭 보기
제품이 사용된 논문 (4)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Theranostics 15:4673-4692 PubMed40225565
2025
Applications
Unspecified application
Species
Unspecified reactive species
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305842 PubMed37967351
2023
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of experimental medicine 220: PubMed36828390
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cancer cell 39:423-437.e7 PubMed33450198
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com