AB169804

Human Inflammation Antibody Array - Membrane (20 targets)

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ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples.

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Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Human peripheral blood cells (1x10⁶ cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.

Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x10⁶ cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804.

Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Quantification of Human Inflammation Antibody Array.

Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Quantification of Human Inflammation Antibody Array.

Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

주요 정보

Reacts with

Human

타겟

CCL1

target

Applications

Multiplex Protein Detection

applications

샘플 타입

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples. Suitable for all liquid sample types.

Targets: Eotaxin-1, Eotaxin-2, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, I-309, TIMP-2

If you are interested in this cytokine array, arrays ab133996, ab133997, ab169817, ab133998 and ab134003 may also be of interest. A list of human antibody membrane arrays is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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제품 구성

{ "values": { "1x8Membranes": { "sellingSize": "1 x 8 Membranes", "publicAssetCode":"ab169804-1x8Membranes", "assetComponentDetails": [ { "size":"1 x 16 mL", "name":"2X Cell Lysis Buffer", "number":"AB169804-CMP09" }, { "size":"2 x 25 mL", "name":"1X Blocking Buffer", "number":"AB169804-CMP03" }, { "size":"1 x 20 mL", "name":"20X Wash Buffer I", "number":"AB169804-CMP07" }, { "size":"1 x 2.5 mL", "name":"Detection Buffer C", "number":"AB169804-CMP05" }, { "size":"1 x 1 Unit", "name":"8-Well Incubation Tray (with Lid)", "number":"AB169804-CMP10" }, { "size":"1 x 20 mL", "name":"20X Wash Buffer II", "number":"AB169804-CMP08" }, { "size":"1 x 2.5 mL", "name":"Detection Buffer D", "number":"AB169804-CMP06" }, { "size":"1 x 1 Unit", "name":"Plastic sheets", "number":"AB169804-CMP11" }, { "size":"1 x 50 µL", "name":"1,000X HRP-Conjugated Streptavidin", "number":"AB169804-CMP04" }, { "size":"2 x 4 Unit", "name":"Inflammation Antibody Array Membranes", "number":"AB169804-CMP02" }, { "size":"4 x 1 Vial", "name":"2000X Biotin-Conjugated Anti Cytokines", "number":"AB169804-CMP01" } ] }, "1x4Membranes": { "sellingSize": "1 x 4 Membranes", "publicAssetCode":"ab169804-1x4Membranes", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"2X Cell Lysis Buffer", "number":"AB169804-CMP09", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Detection Buffer C", "number":"AB169804-CMP05", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Detection Buffer D", "number":"AB169804-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"20X Wash Buffer I", "number":"AB169804-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"1X Blocking Buffer", "number":"AB169804-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"8-Well Incubation Tray (with Lid)", "number":"AB169804-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"20X Wash Buffer II", "number":"AB169804-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plastic sheets", "number":"AB169804-CMP11", "productcode":"" }, { "size":"1 x 50 µL", "name":"1,000X HRP-Conjugated Streptavidin", "number":"AB169804-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"2000X Biotin-Conjugated Anti Cytokines", "number":"AB169804-CMP01", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Inflammation Antibody Array Membranes", "number":"AB169804-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

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제품 프로토콜

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타겟 정보

Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.

See full target information CCL1

Additional targets

CCL11,CCL24,IL1A,IL1B,IL2,IL3,IL6,IL7,TIMP2,IL12B,CSF2,CSF3,CXCL8,IFNG,IL10,IL11,IL12A,IL13

대체 명칭 보기

SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11, IL 11, Interleukin 11, NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40, Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3, Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2, IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin, SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11, MPIF2, SCYA24, CCL24, C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, Myeloid progenitor inhibitory factor 2, Small-inducible cytokine A24, Eotaxin-2, MPIF-2, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF, Interferon gamma, IFN-gamma, Immune interferon, IFNG, IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, NC30, IL13, Interleukin-13, IL-13, Interleukin-7, IL-7, IL7, IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Inflammation Antibody Array - Membrane (20 targets)

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (AB169804)

주요 정보

Reacts with

타겟

Applications

샘플 타입

보완 제품

Reagents, Controls & Accessories

Multiplex Kits

Multiplex Kits

Multiplex Kits

Multiplex Kits

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

Product promise