Western blot reagents
Our western blotting reagents provide reliable and consistent results for protein analysis.
Blocking reagents
Blocking reagents prevent non-specific protein bindings, enhancing signal clarity and sensitivity in western blot assays.
BCA and Bradford protein assays
These assays are important for accurate protein quantitation before western blotting, ensuring experimental precision.
ECL substrates
Enhanced chemiluminescence (ECL) substrates are used for the detection of horseradish peroxidase (HRP) in western blot assyas, allowing for sensitive protein visualization.
InstantBlue® Coomassie protein stain (ISB1L) (ab119211)
- Coomassie InstantBlue® protein stains proven performance: cited in over 595 publications
- Stains at least 40 mini gels per bottle (25 mL/mini gel)
- No gel shrinkage or protein methylation/acetylation
- High sensitivity - detects protein levels as low as 5 ng per band
- Compatible with Mass Spectrometry
button-primary
What is western blotting?
Western blot is a critical technique in molecular biology for the detection and quantification of specific proteins within a complex sample. This method relies on a suite of specialized reagents that play crucial roles at various stages of the procedure. Our western blot reagents offer researchers a range of high-quality tools designed to improve the clarity, sensitivity, and reliability of their results.
Learn more in our intro to western blot.
Classification of western blotting
The classification of western blot results typically involves three key components: molecular weight, band intensity, and specificity.
Molecular weight is determined by comparing the migration of protein bands to a standard ladder, allowing identification of the target protein.
Band intensity reflects the relative abundance of the protein, often quantified using densitometry software. Specificity is ensured through the use of primary and secondary antibodies that bind selectively to the target protein.
Western blot protocol
The western blotting procedure is a comprehensive process, encompassing steps such as sample preparation, gel running, transfer, blocking, antibody incubation, and detection. The full procedure is outlined in detail in our western blot protocol. The guide is suitable for both cell culture and tissue samples and supports both chemiluminescent and fluorescent detection systems, making it adaptable to various experimental needs.
Learn about western blot blocking methods.
Our western blot range
Our blocking reagents effectively minimize non-specific binding, crucial for clear and distinct signal detection. For accurate protein measurement prior to blotting, we provide precise BCA and Bradford protein assays. These help determine the correct loading volumes for gels, which is vital for obtaining quantifiable and reproducible results. Additionally, for the visualization phase, our ECL substrates reveal the presence of target proteins with high sensitivity, allowing researchers to detect even low-abundance proteins effectively.
Each product in our western blot range is backed by rigorous testing and validation to ensure performance consistency. This focus on quality aids scientists in obtaining dependable data that can drive forward their research initiatives with confidence. Whether studying complex protein interactions, conducting comparative protein expression studies, or validating antibodies, we offer a comprehensive suite of products tailored for the diverse needs of the scientific community involved in protein research.