AB139486

Aggresome Detection Kit

Name: Aggresome Detection Kit
Brand: Abcam Limited
SKU: AB139486
Rating: 5 (1 reviews)

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(11 제품이 사용된 논문 )

Aggresome Detection kit (ab139486) provides a rapid, specific and quantitative approach to identify inhibitors relevant to neurodegenerative disease in an authentic cellular context.

3 이미지

Fluorescence Microscopy - Aggresome Detection Kit (AB139486)

Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Aggresome Detection Kit (AB139486)

Aggresomes detected by Aggresome Detection Reagent in HeLa cells.

Aggresomes were detected in HeLa cells after overnight incubation with a range of proteasome inhibitors, as observed by fluorescence microscopy.

Functional Studies - Aggresome Detection Kit (AB139486)

FuncS

Lab

Functional Studies - Aggresome Detection Kit (AB139486)

The increase in the fluorescence intensity of the Aggresome Detection Reagent with the addition of MG-132 in Jurkat cells. Jurkat cells were mock induced with 0.08% DMSO (blue line) or induced with 8 μM MG-132 (black line) in a 37°C, 5% CO2 incubator for 24 hours.

FuncS

Supplier Data

Functional Studies - Aggresome Detection Kit (AB139486)

Excitation and fluorescence emission spectra for Aggresome Detection Reagent and Hoechst 33342.

Excitation and fluorescence emission spectra for Aggresome reagent dye, Ex/Em=500/600 nm (panel A, left) and Hoechst 33342, Ex/Em=350/461 nm (panel B, right). All spectra were determined in 1X Assay Buffer.

주요 정보

검출 방식

Fluorescent

샘플 타입

Suspension cells, Adherent cells

분석 유형

Cell-based

분석 플랫폼

Flow cytometer, Fluorescence microscope

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제품 세부 정보

Abcam's Aggresome Detection kit (ab139486) provides a rapid, specific and quantitative approach to identify inhibitors relevant to neurodegenerative disease in an authentic cellular context. This fixed-cell assay doesn't require non-physiological protein mutations or genetically engineered cell lines. The Aggresome Detection Reagent has been validated under a wide range of conditions and with small molecule modulators, demonstrating suitability for screening compounds of potential therapeutic value, and optimized for antibody co-localization studies to identify interactions between aggregated protein cargo and the various proteins implicated in autophagy and aggresome formation. Suggested uses for this kit include:

1. The identification of small molecules that inhibit aggresome formation.

2. Immuno-localization studies between aggregated protein cargo and the various proteins implicated in aggresome formation, such as histone deacetylase 6, parkin, ataxin-3, dynein motor complex and ubiquilin-1.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab139486-100Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"Hoechst 33342 Nuclear Stain", "number":"AB139486-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Assay Buffer", "number":"AB139486-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Proteasome Inhibitor", "number":"AB139486-CMP01", "productcode":"" }, { "size":"1 x 10 µL", "name":"Aggresome Detection Reagent", "number":"AB139486-CMP04", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-80°C

적절한 장기 보관 조건

-80°C

보관 정보

-80°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The aggresome is a cellular structure that functions in the degradation of misfolded proteins. Often considered an organized response to proteotoxic stress the aggresome acts as a storage site for misfolded proteins to facilitate their clearance. Cells typically express aggresomes in response to an overwhelmed proteasome system. While their actual molecular weight varies due to the complex and aggregate nature they often form near the centrosome of mammalian cells near the microtubule-organizing center.

Biological function summary

The aggresome participates in protein quality control within the cell. This complex serves an essential role in cellular homeostasis by sequestering misfolded or aggregated proteins for degradation through autophagy. Once these misfolded proteins get directed to the aggresome cells envelop them in autophagosomes which facilitates their eventual breakdown in lysosomes. The presence of ubiquitin chains often tags proteins targeted for aggresome formation implicating the ubiquitin-proteasome system in this process.

Pathways

Aggresome formation plays a significant role in the cellular stress response. The ubiquitin-proteasome pathway heavily influences this process given its role in tagging proteins for degradation. Aggresomes collaborate with chaperone proteins like heat shock proteins to recognize and transport misfolded proteins. In addition they interface with the autophagic pathway which links aggresomes to LAMP proteins found on lysosomal membranes that mediate protein degradation within lysosomes.

The aggresome formation holds considerable relevance to neurodegenerative diseases like Alzheimer's and Parkinson's. In these conditions protein aggregation becomes a hallmark feature with aggresome components sometimes linked to disease pathogenesis. For instance α-synuclein in Parkinson’s disease can form aggregates that contribute to neurodegeneration. Similarly the protein tau in Alzheimer's disease can aggregate disrupting neuronal function. Aggresome targeting involves these proteins highlighting the critical intersection of protein management and neurodegenerative disease pathology.

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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:5328 PubMed40527892

2025

Aneuploidy-induced proteostasis disruption impairs mitochondrial functions and mediates aggregation of mitochondrial precursor proteins through SQSTM1/p62.

Applications

Unspecified application

Species

Unspecified reactive species

Prince Saforo Amponsah,Jan-Eric Bökenkamp,Olha Kurpa,Svenja Lenhard,Anna Myronova,Daniel Osmar Vega Velazquez,Celina Hirschelmann,Christian Behrends,Johannes M Herrmann,Markus Räschle,Zuzana Storchová

Cell death discovery 11:176 PubMed40234445

2025

Selective molecular inhibition of the HDAC6 ZnF-UBP binding domain impairs multiple myeloma cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Rincón,Isabel F Coira,Antoine Richieu,Fedaa Attana,Muriel Urwyler,Shozeb Haider,Carole Bourquin,Philippe Bertrand,Muriel Cuendet

Journal of virology 98:e0143724 PubMed39480084

2024

Tripartite motif 25 inhibits protein aggregate degradation during PRRSV infection by suppressing p62-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Ren,Qiming Pei,Haoxin Dong,Xuedan Wei,Liangliang Li,Hong Duan,Gaiping Zhang,Angke Zhang

Cell communication and signaling : CCS 22:217 PubMed38570831

2024

The protein phosphatase-2A subunit PR130 is involved in the formation of cytotoxic protein aggregates in pancreatic ductal adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Nguyen,Al-Hassan M Mustafa,Alessa K Leydecker,Melisa Halilovic,Janine Murr,Falk Butter,Oliver H Krämer

Molecular cancer therapeutics 22:1376-1389 PubMed37586844

2023

Radiotherapy-induced Immune Response Enhanced by Selective HDAC6 Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Satish Kumar R Noonepalle,Scott Grindrod,Nima Aghdam,Xintang Li,Maria Gracia-Hernandez,Christian Zevallos-Delgado,Mira Jung,Alejandro Villagra,Anatoly Dritschilo

Cellular and molecular life sciences : CMLS 79:414 PubMed35816252

2022

Siah2-GRP78 interaction regulates ROS and provides a proliferative advantage to Helicobacter pylori-infected gastric epithelial cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pragyesh Dixit,Swathi Shivaram Suratkal,Shrikant Babanrao Kokate,Debashish Chakraborty,Indrajit Poirah,Supriya Samal,Niranjan Rout,Shivaram P Singh,Arup Sarkar,Asima Bhattacharyya

PLoS genetics 18:e1010264 PubMed35771772

2022

PACSIN1 is indispensable for amphisome-lysosome fusion during basal autophagy and subsets of selective autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yukako Oe,Keita Kakuda,Shin-Ichiro Yoshimura,Naohiro Hara,Junya Hasegawa,Seigo Terawaki,Yasuyoshi Kimura,Kensuke Ikenaka,Shiro Suetsugu,Hideki Mochizuki,Tamotsu Yoshimori,Shuhei Nakamura

Veterinary research 51:104 PubMed32811532

2020

The tail domain of PRRSV NSP2 plays a key role in aggrephagy by interacting with 14-3-3ε.

Applications

Unspecified application

Species

Unspecified reactive species

Shengliang Cao,Jiaqi Liu,Guofei Ding,Qingyuan Shao,Bin Wang,Yingchao Li,Jian Feng,Yuzhong Zhao,Sidang Liu,Yihong Xiao

Journal of medical genetics 57:124-131 PubMed31649052

2019

Pharmacologic properties of high-dose ambroxol in four patients with Gaucher disease and myoclonic epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon-Myung Kim,Mi-Sun Yum,Sun Hee Heo,Taeho Kim,Hee Kyung Jin,Jae-Sung Bae,Go Hun Seo,Arum Oh,Hee Mang Yoon,Hyun Taek Lim,Hyo-Won Kim,Tae-Sung Ko,Hyeong-Seok Lim,Mark J Osborn,Jakub Tolar,Claudia Cozma,Arndt Rolfs,Ari Zimran,Beom Hee Lee,Han-Wook Yoo

Molecular biology of the cell 30:1272-1284 PubMed30893019

2019

Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxin Liu,Xiaowen Wang,Liam P Coyne,Yuan Yang,Yue Qi,Frank A Middleton,Xin Jie Chen

제품이 사용된 논문 모두 보기

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Product promise

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자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Aggresome Detection Kit

Fluorescence Microscopy - Aggresome Detection Kit (AB139486)

Functional Studies - Aggresome Detection Kit (AB139486)

Functional Studies - Aggresome Detection Kit (AB139486)

주요 정보

검출 방식

샘플 타입

분석 유형

분석 플랫폼

보완 제품

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (11)

Product promise