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AB273276

Aldose Reductase Activity Kit (Colorimetric)

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(3 제품이 사용된 논문 )

Aldose Reductase Activity Assay Kit (Colorimetric) (ab273276) uses the ability of Aldose Reductase (AR) to catalyze the oxidation of NADPH.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

대체 명칭 보기

ALDR1, ALR2, AKR1B1, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AR, AKR1B11, AKR1B10, Aldo-keto reductase family 1 member B10, ARL-1, Aldose reductase-like, Aldose reductase-related protein, Small intestine reductase, ARP, hARP, SI reductase, ALDR1, ALR, AKR1A1, Aldo-keto reductase family 1 member A1, Alcohol dehydrogenase [NADP(+)], Aldehyde reductase, Glucuronate reductase, Glucuronolactone reductase, S-nitroso-CoA reductase, ScorR

3 이미지
Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)
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Supplier Data

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)

Purified Aldose Reductase activity.

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)
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Supplier Data

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)

NADPH standard curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)
  • FuncS

Supplier Data

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)

Aldose Reductase Activity in Tissue Samples.

Aldose Reductase specific activity was calculated in Rat Kidney tissue lysates (20 μg protein) and HepG2 cell lysates (110 μg protein).

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue, Suspension cells, Adherent cells

분석 유형

Enzyme activity

결과 유형

Quantitative

Sensitivity

= 100 µU

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Aldose Reductase Activity Assay Kit (Colorimetric) (ab273276) utilizes the ability of Aldose Reductase (AR) to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. The assay has been optimized to be monitored using a 96 well plate. The kit can detect as low as 0.1 mU. The assay kit is simple and can be used in a high-throughput format.

This product is manufactured by BioVision, an Abcam company and was previously called K369 Aldose Reductase Activity Kit (Colorimetric). K369-100 is the same size as the 100 test size of ab273276.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273276-100Test", "assetComponentDetails": [ { "size":"1 x 35 mL", "name":"AR Assay Buffer", "number":"AB273276-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"AR Substrate", "number":"AB273276-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well UV Transparent Plate", "number":"AB273276-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADPH V", "number":"AB273276-CMP04", "productcode":"" }, { "size":"1 x 400 µL", "name":"DTT I (1M)", "number":"AB273276-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Active Aldose Reductase", "number":"AB273276-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Please refer to protocols
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Aldose reductase also known as AR or ALR2 is an important enzyme in the polyol pathway. It has a molecular mass of about 36 kDa and reduces aldehydes including glucose to their corresponding alcohols. Aldose reductase is expressed widely in various tissues such as kidney lens and retina. The enzyme uses NADPH as a cofactor which is important for its catalytic activity.
Biological function summary

The main function of aldose reductase involves converting glucose to sorbitol a process particularly active under hyperglycemic conditions. It does not form a part of a larger complex but works individually to facilitate this conversion. Sorbitol the product of its enzymatic reaction eventually converts to fructose through the action of sorbitol dehydrogenase. This two-step conversion can contribute to cellular damage due to osmotic stress when sorbitol accumulates.

Pathways

Aldose reductase plays a central role in the polyol pathway. This pathway becomes highly relevant when blood glucose levels are elevated. During such conditions aldose reductase activity increases to convert excess glucose to sorbitol. This activity links it to other proteins like sorbitol dehydrogenase which completes the conversion to fructose.

Aldose reductase has strong connections to diabetic complications specifically diabetic neuropathy and retinopathy. The enzyme’s increased activity under high glucose conditions can lead to osmotic stress and tissue damage. Interactions with other proteins in the metabolic shift associated with diabetes such as sorbitol dehydrogenase underline its involvement. Aldose reductase inhibitors show potential in mitigating these adverse effects by reducing sorbitol accumulation.
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제품 프로토콜

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타겟 정보

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed : 1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed : 12732097, PubMed : 19010934, PubMed : 8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed : 17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed : 21329684).
See full target information AKR1B1

Additional targets

AKR1B10,AKR1A1
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Chemico-biological interactions 392:110905 PubMed38373627

2024

High incidence of sebaceous gland inflammation in aldose reductase-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Mandava,Binhan Pham,Michelle Pedler,Biehuoy Shieh,Hari Gopalakrishnan,J Mark Petrash

Cell reports. Medicine 4:101056 PubMed37257447

2023

Tracer metabolomics reveals the role of aldose reductase in glycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Radenkovic,Anna N Ligezka,Sneha S Mokashi,Karen Driesen,Lynn Dukes-Rimsky,Graeme Preston,Luckio F Owuocha,Leila Sabbagh,Jehan Mousa,Christina Lam,Andrew Edmondson,Austin Larson,Matthew Schultz,Pieter Vermeersch,David Cassiman,Peter Witters,Lesa J Beamer,Tamas Kozicz,Heather Flanagan-Steet,Bart Ghesquière,Eva Morava

Clinical and experimental pharmacology & physiology 49:264-274 PubMed34570396

2021

Propofol suppresses cell proliferation in gastric cancer cells through NRF2-mediated polyol pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Cao,Long Fan,Linkai Li,Jiexian Zhou
제품이 사용된 논문 모두 보기
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Product promise

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