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AB242287

Alkaline Phosphatase Staining Kit (Purple)

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(6 제품이 사용된 논문 )

Alkaline Phosphatase Staining Kit (Purple) (ab242286) uses a convenient method to identify and monitor stem cells by staining for alkaline phosphatase activity.

대체 명칭 보기

ALPPL, ALPPL2, ALPG, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, Placental alkaline phosphatase-like, GCAP, PLAP-like, Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI, PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, AP-TNAP, TNS-ALP, TNSALP, Alkaline phosphatase liver/bone/kidney isozyme, Phosphoamidase, Phosphocreatine phosphatase, ALPL

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Immunocytochemistry - Alkaline Phosphatase Staining Kit (Purple) (AB242287)
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Supplier Data

Immunocytochemistry - Alkaline Phosphatase Staining Kit (Purple) (AB242287)

AP staining of ES Cells.

Murine embryonic stem cells (ES-D3) are maintained in an undifferentiated stage on gelatin-coated dishes in the presence of LIF. AP staining of undifferentiated ES cells was performed as described in the Assay Protocol.

주요 정보

검출 방식

Colorimetric

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보완 제품

Assay Kits

AB83369

Alkaline Phosphatase Assay Kit (Colorimetric)

Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (AB83369)

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Assay Kits

AB284936

Alkaline Phosphatase Staining Kit

Staining - Alkaline Phosphatase Staining Kit (AB284936)

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Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Alkaline Phosphatase Staining Kit (Purple) (ab242286) uses a convenient method to identify and monitor stem cells by staining for alkaline phosphatase activity.

Pluripotent stem cells typically have higher levels of alkaline phosphatase activity than differentiated cell types (although osteoblasts retain this activity). Alkaline phosphatase staining / activity is often used as a biomarker of stem cells.

In this alkaline phosphatase staining kit, alkaline phosphatase acts on a substrate to form a purple colored chemical, producing a purple color in stem cells that is imaged with light microscopy.

The kit can be used with any tissue section or cell type that have alkaline phosphatase activity. It is not compatible with paraffin-embedded sections.

The alkaline phosphatase staining protocol is simple:
- prepare samples (the kit protocol is written for use with ES cells; the kit can be used with any cell or tissues with alkaline phosphatase activity)
- add fixing solution to cells or tissues, incubate for 2 mins, and wash with PBST
- add staining solution and incubate for 15-30 mins
- wash with PBS and image

Alkaline Phosphatase Staining Kit (Red) ab242286 is an alternative to this kit, using an equivalent method, with a different colored dye. Generally, the red kit gives cleaner staining.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab242287-100Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Fixing Solution", "number":"AB242287-CMP02", "productcode":"" }, { "size":"1 x 40 mL", "name":"AP Staining Solution", "number":"AB242287-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alkaline Phosphatase also known as ALP or ALKPHOS is an enzyme that removes phosphate groups from various molecules like nucleotides and proteins. This enzymatic activity requires alkaline conditions with a pH optimum around 8–10. ALP is anchored to the cell membrane and exists in several isoforms each with a mass around 57-70 kDa. Different tissues express specific isoforms such as bone liver kidney and placenta. Osteoblasts for example show high levels of alkaline phosphatase expression often detected by techniques like ALP staining or alkaline phosphatase staining.
Biological function summary

Alkaline phosphatase plays important roles in dephosphorylation processes. Particularly in bone it is associated with osteoblast activity where it is important for bone mineralization. The enzyme is not known to be part of a large protein complex but its activity aids in the conversion of inorganic pyrophosphate into phosphate which promotes the deposition of calcium phosphate in bone. ALP staining and ALP staining osteoblast can be used in laboratory settings for studying the mineralization and function of osteoblasts.

Pathways

Alkaline phosphatase is key in the regulation of the phosphate group levels within the organism particularly in the bone mineralization process. It participates in the vitamin D metabolic pathway which influences calcium and phosphorus homeostasis. Its function has a dynamic relationship with proteins like NPP1 which regulates levels of inorganic pyrophosphate acting counter to alkaline phosphatase's phosphate-producing activity.

Defects in alkaline phosphatase activity are linked to conditions like hypophosphatasia and hyperphosphatasia where mineralization processes are disrupted. In hypophosphatasia decreased ALP activity leads to improper bone formation due to accumulation of inorganic pyrophosphate. This interaction is notable alongside proteins such as tissular nonspecific alkaline phosphatase. On the other hand elevated ALP levels often indicate liver or bone disease pathology where increased enzyme levels reflect altered tissue turnover.
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제품 프로토콜

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타겟 정보

Alkaline phosphatase that can hydrolyze various phosphate compounds.
See full target information ALPG

Additional targets

ALPP,Alkaline phosphatase, tissue-nonspecific isozyme,ALPI
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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in bioengineering and biotechnology 11:1254506 PubMed38033818

2023

Chitin scaffolds derived from the marine demosponge stimulate the differentiation of dental pulp stem cells.

Applications

Unspecified application

Species

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Anna Zawadzka-Knefel,Agnieszka Rusak,Monika Mrozowska,Tomasz Machałowski,Andrzej Żak,Katarzyna Haczkiewicz-Leśniak,Michał Kulus,Piotr Kuropka,Marzenna Podhorska-Okołów,Katarzyna Skośkiewicz-Malinowska

Stem cell research & therapy 14:154 PubMed37280707

2023

Suspension culture improves iPSC expansion and pluripotency phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Nerea Cuesta-Gomez,Kevin Verhoeff,Nidheesh Dadheech,Tiffany Dang,Ila Tewari Jasra,Mario Bermudez de Leon,Rena Pawlick,Braulio Marfil-Garza,Perveen Anwar,Haide Razavy,Patricio Adrián Zapata-Morin,Glen Jickling,Aducio Thiesen,Doug O'Gorman,Michael S Kallos,A M James Shapiro

Human cell 36:1041-1053 PubMed36952210

2023

Bone marrow mesenchymal stem cells derived exosomal Lnc TUG1 promotes bone fracture recovery via miR-22-5p/Anxa8 axis.

Applications

Unspecified application

Species

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Wei Li,Lihong Li,Rui Cui,Xiaoqing Chen,Haifeng Hu,Yuyu Qiu

Materials today. Bio 16:100404 PubMed36065353

2022

Functional importance of coacervation to convert calcium polyphosphate nanoparticles into the physiologically active state.

Applications

Unspecified application

Species

Unspecified reactive species

Werner E G Müller,Meik Neufurth,Ingo Lieberwirth,Shunfeng Wang,Heinz C Schröder,Xiaohong Wang

Frontiers in genetics 12:831420 PubMed35308164

2022

Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongchang Yang,Li Miao,Shuai Chang,Qiuli Zhang,Lijuan Yu,Ping He,Yue Zhang,Weixiao Fan,Jie Liu,Xiaoke Hao

Biomaterials 276:121019 PubMed34325337

2021

Effects of purified exosome product on rotator cuff tendon-bone healing in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Ren,Shuwei Zhang,Yicun Wang,Daniel S Jacobson,Ramona L Reisdorf,Tomoyuki Kuroiwa,Atta Behfar,Steven L Moran,Scott P Steinmann,Chunfeng Zhao
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