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AB284520

alpha-Glucosidase Inhibitor Screening Kit (Colorimetric)

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(3 제품이 사용된 논문 )

The alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) ab284520 is a convenient assay for the screening/characterizing Alpha-Glucosidase inhibitors.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

대체 명칭 보기

Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA

2 이미지
Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520)
  • FuncS

Supplier Data

Functional Studies - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520)

Inhibition of alpha-Glucosidase activity by Acarbose.

α-Glucosidase activity inhibition by Acarbose. IC50 of Acarbose was calculated to be 0.74 ± 0.15 mM. Assay was carried out following the kit protocol.

Other - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520)
  • Other

Supplier Data

Other - alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) (AB284520)

Representative image of alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) ab284520

Components shown from left to right :

- alpha-Glucosidase Positive Control

- alpha-Glucosidase Substrate Mix

- Acarbose

- alpha-Glucosidase Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

주요 정보

검출 방식

Colorimetric

샘플 타입

Inhibitor compounds

분석 유형

Enzyme activity

결과 유형

Quantitative

분석 플랫폼

Microplate (12 x 8 well strips)

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

The alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) ab284520 is a convenient assay for the screening/characterizing Alpha-Glucosidase inhibitors.

How the assay works
The alpha-Glucosidase assay uses the ability of an active Alpha-Glucosidase to cleave a synthetic substrate thus, releasing a chromophore (OD: 410 nm). In the presence of an Alpha-Glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings.

Alpha-Glucosidase assay summary
- Add samples and standards to wells
- Add reaction mix and incubate
- Analyze with microplate reader at OD: 410 nm in kinetic mode for 60 min at room temperature.

Other Notes
This product was previously called K938 Biovision a-Glucosidase Inhibitor Screening Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284520-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"α-Glucosidase Assay Buffer", "number":"AB284520-CMP03", "productcode":"" }, { "size":"1 x 300 µL", "name":"α-Glucosidase Substrate Mix", "number":"AB284520-CMP04", "productcode":"" }, { "size":"1 x 140 µL", "name":"Acarbose", "number":"AB284520-CMP01", "productcode":"" }, { "size":"1 x 4 Vial", "name":"alpha-Glucosidase Positive Control", "number":"AB284520-CMP02", "productcode":"AB325890" } ] } } }

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C

제품 프로토콜

타겟 정보

Essential for the degradation of glycogen in lysosomes (PubMed : 14695532, PubMed : 18429042, PubMed : 1856189, PubMed : 7717400). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed : 29061980).
See full target information Lysosomal alpha-glucosidase

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Marine drugs 22: PubMed39728150

2024

Characterisation of High Alkaline-Tolerant Novel Ulvan Lyase from : Potential Applications of Enzyme Derived Oligo-Ulvan as Anti-Diabetic Agent.

Applications

Unspecified application

Species

Unspecified reactive species

Navindu Dinara Gajanayaka,Eunyoung Jo,Minthari Sakethanika Bandara,Svini Dileepa Marasinghe,Chinmayee Bawkar,Yeon-Ju Lee,Gun-Hoo Park,Chulhong Oh,Youngdeuk Lee

Pharmaceuticals (Basel, Switzerland) 17: PubMed38256924

2024

The Immune-Stimulating and Anti-Diabetic Effects of Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jieun Jung,Ji-Su Kim,Un-Yul Jeong,Ui-Jin Bae,Mina Kim,Shin-Young Park,In-Guk Hwang,Jeong-Wook Heo,Chang-Ki Shim,Jun-Sang Ham,Sung-Hyen Lee

Molecules (Basel, Switzerland) 28: PubMed37513196

2023

Phytofabrication of Selenium Nanoparticles with (MO-SeNPs) and Exploring Its Antioxidant and Antidiabetic Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Anas Ahzaruddin Ahamad Tarmizi,Nik Nasihah Nik Ramli,Siti Hajar Adam,Maisarah Abdul Mutalib,Mohd Helmy Mokhtar,Shirley Gee Hoon Tang
제품이 사용된 논문 모두 보기
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Product promise

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