Aspartate Aminotransferase Activity Assay Kit (Fluorometric)
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Aspartate Aminotransferase Activity Assay Kit / AST Assay Kit (Fluorometric) ab138878 provides a quick and sensitive assay for AST activity in biological samples.
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cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1, KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm
- FuncS
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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (Fluorometric) (AB138878)
Sample Standard Curve for Aspartate Aminotransferase. AST dose response was measured with ab138878 in a 96-well black plate using a fluorescence microplate reader. As low as 2 mU/ml AST can be detected with 60 min incubation (n=3) at room temperature.
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Aspartate Aminotransferase Activity Assay Kit / AST Assay Kit (Fluorometric) ab138878 provides a quick and sensitive assay for AST activity in biological samples.
In the AST assay protocol, AST catalyzes the reaction of aspartate and α-ketoglutarate to oxaloacetate and glutamate:
Aspartate (Asp) + α-ketoglutarate ⇌ oxaloacetate + glutamate (Glu)
The product L-glutamate is measured by the generation of a red fluorescent product through an enzyme coupled reaction cycle.
The assay signal can be read by a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (optimal Ex/Em = 540 nm/590 nm). The AST activity assay can detect as little as 2 mU/mL AST in a 100 μL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.
Aspartate aminotransferase (AST), also called Glutamic Oxaloacetic transaminase (GOT), is a member of transferase family. It catalyzes the reversible transfer of an α-amino group between aspartate and glutamate, and is an important enzyme in amino acid metabolism.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.
Pathways
AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.
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