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AB102512

Aspartate Assay Kit

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(20 제품이 사용된 논문 )

Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.
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Functional Studies - Aspartate Assay Kit (AB102512)
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Supplier Data

Functional Studies - Aspartate Assay Kit (AB102512)

Fluorometric Standard Curve Performed as Described in the Protocol

Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Lab

Functional Studies - Aspartate Assay Kit (AB102512)

Aspartate levels measured fluorometrically in rat biological fluids, background signal subtracted (duplicates +/- SD).

Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Lab

Functional Studies - Aspartate Assay Kit (AB102512)

Aspartate levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Supplier Data

Functional Studies - Aspartate Assay Kit (AB102512)

Colorimetric Standard Curve Performed as Described in the Protocol

Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Lab

Functional Studies - Aspartate Assay Kit (AB102512)

Aspartate levels measured colourometrically in mouse tissue lysates (mg of extracted protein : background signal subtracted, mean of duplicates; +/- SD).

Other - Aspartate Assay Kit (AB102512)
  • Other

Supplier Data

Other - Aspartate Assay Kit (AB102512)

Representative image of Aspartate Assay Kit ab102512

Components shown from left to right :

- OxiRed Probe

- Sample Clean-Up Mix I

- Aspartate Standard

- Aspartate Enzyme Mix

- Converter Mix J

- Assay Buffer 4

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

주요 정보

검출 방식

Colorimetric/Fluorometric

샘플 타입

Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

결과 유형

Quantitative

Sensitivity

> 2 µM

검출 범위

2 - 200 µM

분석 소요 시간

40m

분석 플랫폼

Microplate reader

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제품 세부 정보

Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.

In the aspartate assay protocol, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 μM).

This product is manufactured by BioVision, an Abcam company and was previously called K552 Aspartate Colorimetric/Fluorometric Assay Kit. K552-100 is the same size as the 100 test size of ab102512.

L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys).

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102512-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB102512-CMP03", "productcode":"AB309437" }, { "size":"1 x 0.1 mL", "name":"Aspartate Standard", "number":"AB102512-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Sample Clean-Up Mix I", "number":"AB102512-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix J", "number":"AB102512-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB102512-CMP01", "productcode":"AB323949" }, { "size":"1 x 1 Vial", "name":"Aspartate Enzyme Mix", "number":"AB102512-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate also known as aspartic acid is a non-essential amino acid with a molecular mass of 133.10 g/mol. It is an important component in proteins and is found widely expressed in various tissues throughout the body. Aspartate participates in several metabolic processes acting as a neurotransmitter in the central nervous system and playing roles in enzyme function as a medium for nitrogen transport and as a substrate in the urea cycle.
Biological function summary

Members of the broader aspartate family are important in several biochemical areas. Aspartic acid works as a precursor for the synthesis of other amino acids and nucleotides. It also forms part of aspartate transaminase an enzyme that contributes to the transfer of amino groups. In cellular metabolism aspartate integrates into the synthesis of purines and pyrimidines contributing to nucleotide formation which is critical for DNA and RNA production.

Pathways

The role of aspartate extends to significant metabolic routes. It participates in the citric acid cycle also known as the Krebs cycle where it can be converted into oxaloacetate. It also maps to the urea cycle where it acts to eliminate excess nitrogen from organisms. Through these pathways aspartate interacts with proteins such as fumarase and argininosuccinate synthase highlighting its connectivity to fundamental metabolism.

Abnormalities in aspartate levels can associate with specific medical conditions. For instance elevated aspartate transaminase may indicate liver disease as damaged liver cells release plentiful enzymes into the bloodstream. Furthermore aspartate dysregulation is linked to disorders like hyperammonemia where the protein's failure to connect to glutamate and urea cycle proteins such as ornithine transcarbamylase results in excess ammonia levels in the blood.
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제품 프로토콜

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타겟 정보

See full target information Aspartic Acid
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제품이 사용된 논문 (20)

Recent publications for all applications. Explore the 전체 목록 and refine your search

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Nitrate promotes the growth and the production of short-chain fatty acids and tryptophan from commensal anaerobe in the lactate-deficient environment by facilitating the catabolism of glutamate and aspartate.

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BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity.

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Shan Yan,Changxing Qi,Wei Song,Qianqian Xu,Lianghu Gu,Weiguang Sun,Yonghui Zhang

International journal of radiation oncology, biology, physics 110:1295-1305 PubMed33838214

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Pharmacological Inhibition of HSP90 Radiosensitizes Head and Neck Squamous Cell Carcinoma Xenograft by Inhibition of DNA Damage Repair, Nucleotide Metabolism, and Radiation-Induced Tumor Vasculogenesis.

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