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AB272521

beta-Glucosidase Assay Kit

beta-Glucosidase 분석 키트

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(3 제품이 사용된 논문 )

Beta-Glucosidase Assay Kit (ab272521) is a simple, direct and automation-ready procedure for measuring β-glucosidase activity.
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Enzyme activity assay - beta-Glucosidase Assay Kit (AB272521)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - beta-Glucosidase Assay Kit (AB272521)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Example of beta-Glucosidase standard curve.

Enzyme activity assay - beta-Glucosidase Assay Kit (AB272521)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - beta-Glucosidase Assay Kit (AB272521)

Example of beta-Glucosidase reaction progress curves.

주요 정보

검출 방식

Colorimetric

결과 유형

Quantitative

검출 범위

2 - 250 mU/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Beta-Glucosidase Assay Kit (ab272521) is a simple, direct and automation-ready procedure for measuring β-glucosidase activity. beta-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.

Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.

Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab272521-100Test", "assetComponentDetails": [ { "size":"1 x 24 mL", "name":"Assay Buffer", "number":"AB272521-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"β-NPG Substrate", "number":"AB272521-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Calibrator", "number":"AB272521-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Beta-glucosidase also known as β-glucosidase or GCase is an enzyme that catalyzes the hydrolysis of β-glucosidic bonds in β-glucosides releasing glucose. This enzyme has a molecular mass of around 55 kDa and occurs in various organisms including plants microorganisms and humans. In humans beta-glucosidase is expressed in tissues such as the spleen liver and kidney. The protein plays a mechanical role in cleaving glucose from different glycosides which is important for various metabolic processes.
Biological function summary

Beta-glucosidase functions by aiding the degradation of complex carbohydrates facilitating the release of glucose for cellular energy use and material recycling. This enzyme is not typically part of a large molecular complex but operates independently to fulfill its function. Its activity is essential for breaking down plant-derived compounds and is significant in the metabolic processes that contribute to energy production and detoxification of harmful glucosides.

Pathways

Beta-glucosidase participates in the glycosphingolipid metabolism and cellulose degradation pathways. In glycosphingolipid metabolism it is involved in the lysosomal pathway where it breaks down glucosylceramide into ceramide and glucose. This activity impacts protein networks that include lysosomal enzymes. In cellulose degradation beta-glucosidase acts as a final step converting cellobiose into two glucose molecules linking it to other carbohydrate-active enzymes like cellulase.

Beta-glucosidase deficiency is connected to Gaucher's disease a lysosomal storage disorder where glucosylceramide accumulates due to insufficient breakdown. This enzyme's dysfunction also indirectly impacts proteins involved in lysosomal function contributing to disease pathology. Additionally beta-glucosidase may associate with Parkinson's disease through its role in cellular metabolism and the energy dysfunction observed in neurodegenerative conditions. The protein interacts with other metabolic and lysosomal enzymes indicating its broader involvement in health and disease contexts.
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제품 프로토콜

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타겟 정보

Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides (PubMed : 11389701, PubMed : 11784319, PubMed : 17595169, PubMed : 20728381, PubMed : 26724485, PubMed : 33361282). Has a significant glucosylceramidase activity in vitro (PubMed : 17595169, PubMed : 26724485). However, that activity is relatively low and its significance in vivo is not clear (PubMed : 17595169, PubMed : 20728381, PubMed : 26724485). Hydrolyzes galactosylceramides/GalCers, glucosylsphingosines/GlcSphs and galactosylsphingosines/GalSphs (PubMed : 17595169). However, the in vivo relevance of these activities is unclear (PubMed : 17595169). It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics (PubMed : 11784319). Possesses transxylosylase activity in vitro using xylosylated ceramides/XylCers (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) as xylosyl donors and cholesterol as acceptor (PubMed : 33361282). Could also play a role in the catabolism of cytosolic sialyl free N-glycans (PubMed : 26193330).
See full target information GBA3

대체 명칭 보기

CBG, CBGL1, GBA3, Cytosolic beta-glucosidase, Cytosolic beta-glucosidase-like protein 1, Cytosolic galactosylceramidase, Cytosolic glucosylceramidase, Cytosolic glycosylceramidase, Glucosidase beta acid 3, Glucosylceramidase beta 3, Klotho-related protein, Cytosolic GCase, KLrP
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Neuromolecular medicine 27:42 PubMed40397198

2025

Protective Effect of the LRRK2 Kinase Inhibition in Human Fibroblasts Bearing the Genetic Variant GBA1 K198E: Implications for Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Patricia Perez-Abshana,Miguel Mendivil-Perez,Carlos Velez-Pardo,Marlene Jimenez-Del-Rio

International journal of molecular sciences 25: PubMed39273169

2024

The GBA1 K198E Variant Is Associated with Suppression of Glucocerebrosidase Activity, Autophagy Impairment, Oxidative Stress, Mitochondrial Damage, and Apoptosis in Skin Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Patricia Perez-Abshana,Miguel Mendivil-Perez,Marlene Jimenez-Del-Rio,Carlos Velez-Pardo

International journal of molecular sciences 24: PubMed37445771

2023

Rotenone Blocks the Glucocerebrosidase Enzyme and Induces the Accumulation of Lysosomes and Autophagolysosomes Independently of LRRK2 Kinase in HEK-293 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Patricia Perez-Abshana,Miguel Mendivil-Perez,Carlos Velez-Pardo,Marlene Jimenez-Del-Rio
제품이 사용된 논문 모두 보기
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