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AB325214

BOND RX - Anti-STAT5 (phospho Y694) antibody [E208]

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Rabbit Recombinant Monoclonal antibody detecting STAT5 in Western Blot, IHC-P, ELISA. Suitable for Human. This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-STAT5 (phospho Y694) antibody [E208] - ab32364.

- Validated on the Leica BOND RX automated IHC staining platform for STAT5 IHC
- E208 clone cited in over 70 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOND RX - Anti-STAT5 (phospho Y694) antibody [E208] (AB325214)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOND RX - Anti-STAT5 (phospho Y694) antibody [E208] (AB325214)

This data was developed using the same antibody clone in a different buffer formulation containing PBS only (ab219607).

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling STAT5 (phospho Y694) with ab219607 [E208] at 0.02 µg/mL concentration.

All steps were performed at room temperature unless otherwise stated.

Staining was performed on the BOND RX Advanced Staining Instrument from Leica Biosystems, using the Bond Polymer Refine Detection kit (DS9800) and the following protocols :

  1. Deparaffinization - Dewax
  2. Antigen retrieval - HIER with ER2 (pH 9.0), 20 minutes, 100°C
  3. Staining – modified IHC Protocol F (Marker : ab219607 for 15 minutes with 3 x Bond Wash Solution steps) where standard post-primary step has been excluded.

Sections were counterstained with Hematoxylin (DS9800).

Secondary Antibody only control was performed with the same staining protocols, with marker as negative.

주요 정보

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보완 제품

Primary Antibodies

AB32043

Anti-STAT5a antibody [E289]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (AB32043)

  • 5
  • 2
Primary Antibodies

AB314033

Anti-STAT1 (phospho S727) antibody [2H10]

BOND RX™ Validated|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 (phospho S727) antibody [2H10] (AB314033)

  • 0
  • 0
Primary Antibodies

AB267373

Anti-STAT3 (phospho Y705) antibody [EPR23968-52]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-STAT3 (phospho Y705) antibody [EPR23968-52] (AB267373)

  • 0
  • 0
Primary Antibodies

AB178941

Anti-STAT5b antibody [EPR16671]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (AB178941)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.02 µg/mL", "notes":"<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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제품 세부 정보

This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-STAT5 (phospho Y694) antibody [E208] - ab32364.

Additional Titration Inserts can be purchased from Leica Biosystems, using OPT9719 (box of 50). Or Titration Container/Insert Kit, using OPT9049 (box of 10/50).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab325214-1Kit", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"LBS Rx BOND Titration Container", "number":"AB325214-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Anti-STAT5 (phospho Y694) antibody [E208]", "number":"AB325214-CMP02", "productcode":"AB32364" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
See full target information STAT5A pY694

대체 명칭 보기

STAT5, STAT5A, Signal transducer and activator of transcription 5A
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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