AB138880

cAMP Assay Kit (Competitive ELISA, Fluorometric)

cAMP 분석 키트 (Competitive ELISA, Fluorometric)

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cAMP Assay Kit ab138880 is a competitive ELISA used to quantify cAMP with a sensitivity of 1nM

- Fluorometric readout - Ex/Em 540/590 nm
- no acetylation step required

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ELISA - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

ELISA

Lab

ELISA - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

Immunoassay - ab13880cAMP Direct Immunoassay Kit (fluorometric).

cAMP concentration (nM) measured in various biological fluids following assay protocol.

Functional Studies - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

FuncS

Supplier Data

Functional Studies - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

Sample Standard Curve for cAMP. cAMP dose response was measured with ab138880 in a solid black 96-well plate with a fluorescence microplate reader. The kit can detect as low as 1 nM cAMP in a 100 µl reaction volume.

ELISA

Lab

ELISA - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

cAMP dose response in various amounts of 3T3-L1 cells after and without forskolin treatment (100 micromolar - ab120058)

ELISA

Lab

ELISA - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

Immunoassay - 138880 cAMP Direct Immunoassay Kit (Fluorometric).

cAMP dose response in various amounts of HeLa cells after and without forskolin treatment (100 micromolar - ab120058)

주요 정보

검출 방식

Fluorescent

샘플 타입

Urine, Plasma, Tissue Extracts, Cell culture media, Cell Lysate

분석 유형

Competitive

Sensitivity

= 1 nM

분석 소요 시간

분석 플랫폼

Microplate reader

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제품 세부 정보

cAMP Assay Kit (Competitive ELISA, Fluorometric) ab138880 measures cyclic AMP activity in cell extracts, tissue extracts and other biological fluids with a sensitivity of 1nM.

How the cAMP assay works

The cAMP assay is based on the competition between HRP-labeled cAMP and free cAMP present in the sample for cAMP antibody binding sites.

In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. Free cAMP (unlabeled) present in the test sample competes with HRP-cAMP for anti-cAMP antibody, therefore displacing HRP-cAMP and inhibiting its binding to the antibody.

The fluorescence observed (Ex/Em = 540/590 nm) is proportional to the activity of HRP-cAMP conjugate: the more cAMP present in the test samples, the lower the fluorescence detected.

cAMP assay protocol summary

- add standards and samples to appropriate wells

- add HRP-cAMP conjugate to samples & standards

- incubate RT 2 hours

- wash wells

- add detector (AbRed Working solution)

- incubate RT 15 – 60 minutes

- measure fluorescence (Ex/Em = 540/590 nm)

How other researchers have used cAMP assay kit ab138880

cAMP Assay Kit ab138880 has been used in a variety of sample types:

- HEK293T cells (1)

- isolated cardiac mitochondria (2)

- sperm samples (3)

References: 1 - Ruiqi Liu et al. 2023; 2 - Maura Greiser et al. 2023; 3 - Qifu He et al. 2023

Related and recommended products

cAMP is commonly used to study signal transduction pathways. Other kits to measure cAMP include:

- cAMP Assay Kit (Competitive ELISA, Fluorometric) ab323510, and cAMP ELISA Kit - Intracellular ab323511.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

Multi

적절한 장기 보관 조건

Multi

보관 정보

Please refer to protocols

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cyclic adenosine monophosphate (cAMP) serves as an essential secondary messenger in various signal transduction pathways. Known also by its chemical abbreviation cAMP this small molecule has a mass of approximately 329.21 g/mol. cAMP is synthesized from ATP by the enzyme adenylate cyclase which is present in many cell types notably in neurons and various endocrine tissues. cAMP acts by activating protein kinase A (PKA) which then phosphorylates target proteins to alter cellular function.

Biological function summary

CAMP regulates numerous cellular processes including metabolism gene expression and cell growth. It functions as an important part of G protein-coupled receptor (GPCR) signaling pathways where its concentration can significantly change influenced by external signals. cAMP modulates the activity of ion channels and various enzymes often participating in feedback loops to maintain cellular homeostasis.

Pathways

CAMP integrates into critical signal transduction pathways such as the cyclic AMP-dependent pathway and the calcium signaling pathway. It works closely with proteins like CREB (cAMP response element-binding protein) to regulate gene transcription. cAMP also impacts other molecules like phosphodiesterases which break it down maintaining its transient nature in response to stimuli.

Irregular cAMP levels associate with pathologies like heart failure and certain types of cancer. Disruptions in cAMP signaling can lead to abnormal cell growth or inadequate cellular response to hormonal signals. Through its interaction with proteins like CFTR (cystic fibrosis transmembrane conductance regulator) cAMP influences disorders such as cystic fibrosis highlighting its critical role in maintaining cellular function and signaling balance.

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제품 프로토콜

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타겟 정보

See full target information Cyclic AMP

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제품이 사용된 논문 (26)

Recent publications for all applications. Explore the 전체 목록and refine your search

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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cAMP Assay Kit (Competitive ELISA, Fluorometric)

cAMP 분석 키트 (Competitive ELISA, Fluorometric)

ELISA - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

Functional Studies - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

ELISA - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

ELISA - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

주요 정보

검출 방식

샘플 타입

분석 유형

Sensitivity

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Reagents, Controls & Accessories

Assay Kits

Secondary Antibodies

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (26)

Product promise