Cell Viability Assay Kit (Fluorometric - Blue)

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Abcam Cell Viability Assay Kits are a set of tools for monitoring cell viability and cellular functions. There are a variety of parameters that can be used to monitor cell viability.

ab112120 uses our proprietary cell viability dye whose fluorescence is strongly enhanced upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The weakly fluorescent Blue probe is hydrolyzed by intracellular esterase to generate a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells, and thus directly related to the fluorescence intensity of the product generated from the esterase-catalyzed hydrolysis of the fluorogenic substrate. Cells grown in black wall/clear bottom plates can be stained and quantified in less than two hours.

ab112120 is more robust than tetrazolium salt based assays. It can be readily adapted for many different types of fluorescence platforms such as microplate assays, fluorescence microscope, and flow cytometry.

ab112120 is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It provides all the essential components with an optimized cell-labeling protocol and can be used for both suspension and adherent cells.

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Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.