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AB242300

Cellular Antioxidant Assay Kit

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Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.
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Functional Studies (Act) - Cellular Antioxidant Assay Kit (AB242300)
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Supplier Data

Functional Studies (Act) - Cellular Antioxidant Assay Kit (AB242300)

Cellular Antioxidant Activity of Quercetin in HeLa Cells.

60,000 HeLa cells were seeded and cultured in a 96-well plate until confluent. Cells were then pretreated with DCFH-DA and Quercetin for 60 minutes at 37°C. Free Radical Initiator was then added to the cells to begin the assay, which was read every five minutes for 1 hour at 37°C.

Functional Studies - Cellular Antioxidant Assay Kit (AB242300)
  • FuncS

Supplier Data

Functional Studies - Cellular Antioxidant Assay Kit (AB242300)

Dose-Response Curve of Quercetin Standard

Functional Studies - Cellular Antioxidant Assay Kit (AB242300)
  • FuncS

Supplier Data

Functional Studies - Cellular Antioxidant Assay Kit (AB242300)

Example Data for Calculation of AUC for Antioxidant Sample and Negative Control

주요 정보

검출 방식

Fluorescent

샘플 타입

Adherent cells

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제품 세부 정보

Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.

Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

Fluorescence is measured over time in a standard microplate fluorometer. This fluorescence correlates to the Quercetin's ability to quench free radicals. Test antioxidant values can be compared to Quercetin to determine antioxidant activity within the cell.

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제품 구성

{ "values": { "192Test": { "sellingSize": "192 Test", "publicAssetCode":"ab242300-192Test", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"96-Well Cell Culture Microtiter Plate", "number":"AB242300-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"DCFH-DA Probe (1000X)", "number":"AB242300-CMP02", "productcode":"" }, { "size":"1 x 250 µL", "name":"Quercetin", "number":"AB242300-CMP04", "productcode":"" }, { "size":"1 x 200 mg", "name":"Free Radical Initiator", "number":"AB242300-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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제품 프로토콜

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타겟 정보

See full target information Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nanomaterials (Basel, Switzerland) 13: PubMed36903823

2023

An Analysis of the Toxicity, Antioxidant, and Anti-Cancer Activity of Cinnamon Silver Nanoparticles in Comparison with Extracts and Fractions of Cinnamomum Cassia at Normal and Cancer Cell Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Y G El-Baz,A Moustafa,M A Ali,G E El-Desoky,S M Wabaidur,M M Faisal
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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