Chitotriosidase Assay Kit ab241009 uses a fluorogenic substrate that can be hydrolyzed by chitinase and a set of proprietary assay buffers that can distinguish specific CHIT1 activity from other hydrolases including Acidic Mammalian Chitinase.
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Chitotriosidase-1, Chitinase-1, CHIT1
- FuncS
Supplier Data
Functional Studies - Chitotriosidase Assay Kit (AB241009)
Measurement of CHIT1 activity in Human Plasma (4 μL), Human Serum (4 μL), U937 Cell Extract (10 μg) and J774 Cell Extract (10 μg). All assays were performed following kit protocol.
- FuncS
Supplier Data
Functional Studies - Chitotriosidase Assay Kit (AB241009)
4-Methylumbelliferon Standard Curve.
- FuncS
Supplier Data
Functional Studies - Chitotriosidase Assay Kit (AB241009)
Measurement of purified Human Chitiotriosidase activity with or without Inhibition Buffer
- Other
Supplier Data
Other - Chitotriosidase Assay Kit (AB241009)
Representative image of Chitotriosidase Assay Kit ab241009
Components shown from left to right :
- Chitinase Substrate
- 4-Methyumbelliferone Standard
- Chitotriosidase
- CHIT1 Inhibition Buffer
- Assay Buffer 51
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
보완 제품
Reactivity 정보
제품 세부 정보
Chitotriosidase Assay Kit (ab241009) utilizes a fluorogenic substrate that can be hydrolyzed by chitinase and a set of proprietary assay buffers that can distinguish specific CHIT1 activity from other hydrolases including Acidic Mammalian Chitinase. The kit provides a simple, specific, sensitive assay that can detect as low as 0.2 mU/mL of CHIT1 in a variety of biological samples.
Other Notes
This product was previously called K512 Chitotriosidase Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CHIT1 participates in inflammatory processes and helps in tissue remodeling which is important in immune responses. CHIT1 does not typically form part of a complex but operates independently to mediate macrophage response through its chitinase activity. It handles polysaccharide elements in cellular debris impacting macrophage activation states. Macrophages utilize CHIT1 to counteract foreign bodies engulfed during phagocytosis playing essential roles in managing fungal infections.
Pathways
CHIT1 influences both the immune response and metabolic pathways through its enzyme function. One key pathway involving CHIT1 is the mannose receptor-mediated clearance pathway which clears glycoproteins from systemic circulation. CHIT1 activity mirrors that of other chitinases like AMCase although they derive from separate genetic backgrounds. CHIT1 contribution enhances macrophage functionality within phagolysosomal pathways important for broad-spectrum pathogen eradication.
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com