Creatinine Assay Kit (Colorimetric)

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Creatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine.

Creatine assay method
Creatinine Assay Kit ab204537 is based upon the Jaffe reaction. Creatinine reacts with alkaline picrate to produce an orange color. The color intensity is measured before and after adding acid, and the difference is proportional to the creatinine concentration.

Creatinine Assay protocol summary:
- Add samples to wells
- Add reaction mix and incubate for 30 minutes at room temperature
- Measure optical density (OD490 nm)

Related and recommended products
See below other alternative Creatinine Assay Kits:
- Creatinine Assay Kit ab65340
- Albumin Creatinine Ratio Assay Kit ab241018
- Protein Creatinine Ratio Assay Kit ab272539

Other Notes
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted nonenzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.