AB242293

Diacylglycerol Assay Kit

Diacylglycerol 분석 키트

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(11 제품이 사용된 논문 )

ab242293 measures diacylglycerol (DAG) content in samples by a coupled enzymatic reaction system.

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Functional Studies - Diacylglycerol Assay Kit (AB242293)

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Supplier Data

Functional Studies - Diacylglycerol Assay Kit (AB242293)

DAG Detection of Lipid Extracts.

HEK293 and COS-7 lipid extracts were prepared according to the extraction procedure above. DAG samples were tested according to the Assay Protocol (phosphatidic acid background was determined +/- Kinase Mixture). Negative control values (without DAG) have been subtracted.

FuncS

Supplier Data

Functional Studies - Diacylglycerol Assay Kit (AB242293)

DAG Assay Standard Curve.

DAG standard curve was performed according to the Assay Protocol. Background has been subtracted.

주요 정보

검출 방식

Fluorescent

샘플 타입

Cell Lysate

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제품 세부 정보

ab242293 measures diacylglycerol (DAG) content in samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG samples, yielding phosphatidic acid. Next, a lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Finally, the glycerol-3- phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide which reacts with the kit's Fluorometric Probe (Ex. 530-560 nm/Em. 585-595 nm).

ab242293 is a simple, fluorometric assay that quantitatively measures total DAG in cell lysates using a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. The kit contains a DAG Standard and has a detection sensitivity limit of ~15 uM.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab242293-100Test", "assetComponentDetails": [ { "size":"3 x 1.4 mL", "name":"Lipase Solution", "number":"AB242293-CMP06", "productcode":"" }, { "size":"3 x 1.75 mL", "name":"Enzyme Mixture", "number":"AB242293-CMP03", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"10X Assay Buffer", "number":"AB242293-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"DAG Standard", "number":"AB242293-CMP02", "productcode":"" }, { "size":"1 x 110 µL", "name":"Fluorometric Probe", "number":"AB242293-CMP04", "productcode":"" }, { "size":"2 x 1 mL", "name":"Kinase Mixture", "number":"AB242293-CMP05", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-80°C

적절한 장기 보관 조건

-80°C

보관 정보

-80°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Diacylglycerol (DAG) is a lipid molecule that plays a mechanical role as a secondary messenger in cellular processes. It is sometimes referred to as DAG lipid or diacylglycerols. DAG consists of two fatty acid chains bonded to a glycerol backbone. DAG is expressed in the cell membrane and cytoplasm across various tissues. The mass of this molecule varies depending on the length and saturation of its fatty acid chains which can impact its specific role and function in different biological contexts.

Biological function summary

Diacylglycerol participates in signal transduction pathways by activating protein kinase C (PKC) an important component in cellular signaling. DAG does not typically function alone; it often acts within multiprotein complexes influencing various cellular responses through modulation of PKC activity. Through these interactions DAG regulates processes like cell growth differentiation and apoptosis.

Pathways

DAG is a central component in the phosphoinositide pathway and the mitogen-activated protein kinase (MAPK) pathway. In the phosphoinositide pathway it works alongside inositol trisphosphate (IP3) to transduce extracellular signals into cellular responses. In the MAPK pathway DAG aids in the activation of proteins that play roles in proliferation and stress response. Related proteins include phospholipase C which produces DAG by hydrolyzing phosphatidylinositol 45-bisphosphate and PKC which is activated by DAG to propagate intracellular signaling.

DAG is linked to conditions such as cancer and obesity. Dysregulation of DAG levels can lead to uncontrolled cell proliferation contributing to tumorigenesis. In obesity altered DAG signaling may impair insulin sensitivity affecting glucose metabolism. In these contexts the protein kinase C family is often implicated due to DAG's role in PKC activation making DAG a target of interest in disease modulation and treatment strategies.

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제품 프로토콜

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타겟 정보

See full target information Diacylglycerol

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제품이 사용된 논문 (11)

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Diacylglycerol Assay Kit

Diacylglycerol 분석 키트

Functional Studies - Diacylglycerol Assay Kit (AB242293)

Functional Studies - Diacylglycerol Assay Kit (AB242293)

주요 정보

검출 방식

샘플 타입

보완 제품

Primary Antibodies

Secondary Antibodies

Assay Kits

Assay Kits

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (11)

Product promise