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AB176741

Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation)

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Abcam's Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry.
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Flow Cytometry - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)

Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741). Jurkat cells were treated and stained by Blue Viability Dye. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry as described above. Live (Green) and Dead (heat-treated, Red) cells were distinguished with Pacific Blue channel. The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.

Flow Cytometry - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)

Excitation and Emission spectra for CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue) (ab176741)

Spectrum for ab176741.

Fluorescence Microscopy - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)

Fluorescent analysis of HeLa cells fixed with formaldehyde and labeled with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue) in a Costar black walled/clear bottom 96-well plate.

주요 정보

검출 방식

Fluorescent

샘플 타입

Suspension cells, Adherent cells

분석 유형

Cell-based

결과 유형

Quantitative

분석 플랫폼

Flow cytometer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

Abcam's Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 folds and can be completely preserved after fixation.

Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (ab176741) is designed to label cells at Ex/ Em =353/442 nm excited with a laser at 335 nm.

Related assays

Review the cell health assay guide to learn about kits to perform a cell viability assaycytotoxicity assay and cell proliferation assay.

제품 구성

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176741-200Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Tracking dye Blue", "number":"AB176741-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"DMSO", "number":"AB176741-CMP01", "productcode":"" } ] } } }

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C

제품 프로토콜

타겟 정보

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Product promise

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