GSH+GSSG / GSH Assay Kit (Colorimetric)

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GSH+GSSG / GSH Assay Kit (Colorimetric) (ab239709) provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader.

The assay is based on the glutathione recycling system by DTNB and glutathione reductase. DTNB and glutathione (GSH) react to generate 2-nitro-5-thiobenzoic acid which has a yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412 nm.

The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. Therefore, the recycling system dramatically improves the sensitivity of total glutathione detection.

The kit includes the 5-Sulfosalicylic acid (SSA) for the removal of proteins from samples and for the protection of GSH oxidation and g-glutamyl transpeptidase reaction.

The kit can quantify glutathione from 1-100 ng/well in a 200 µL reaction. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will generate stronger signal.

The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutathione.

This product is manufactured by BioVision, an Abcam company and was previously called K261 Glutathione Colorimetric Assay Kit. K261-100 is the same size as the 100 test size of ab239709.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.