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AB115062

Histone H3 (di-methyl K9) Quantification Kit (Colorimetric)

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Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as in heterochromatin formation.

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9me2, H3K9me, H3K9

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Functional Studies - Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (AB115062)
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Supplier Data

Functional Studies - Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (AB115062)

Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476) and the amount of dimethyl-H3-K9 was measured using ab115062.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

결과 유형

Quantitative

분석 소요 시간

2h 30m

분석 플랫폼

Microplate reader

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보완 제품

Assay Kits

AB115056

Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric)

Functional Studies - Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (AB115056)

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Assay Kits

AB115072

Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric)

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Primary Antibodies

AB194688

Anti-Histone H3 (mono methyl K27) antibody

Western blot - Anti-Histone H3 (mono methyl K27) antibody (AB194688)

  • 2
  • 1
Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

  • 4
  • 6
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제품 세부 정보

Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as in heterochromatin formation. Specifically, H3 (di-methyl K9) mediates heterochromatin formation by forming a binding site for HP1.

Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062) allows the user to specifically measure global di-methylation of histone H3K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
Multi
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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제품 프로토콜

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 di methyl K9
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websiteProtocolBooklet
en
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Product promise

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