JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB115124

Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)

4

(1 리뷰)

|

(9 제품이 사용된 논문 )

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.
3 이미지
Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)
  • FuncS

Lab

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)

Standard curve with background signal subtracted (duplicates; +/- SD).

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)
  • FuncS

Unknown

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)

The differences in H3 acetylation level in fetal livers (female fetuses aged 19 dpc) from F1, F2, and F3 generations. measured with ab113476. Histones were prepared using ab115124
Asterisk indicate a significant difference between the respective C and R groups : ∗∗ P < 0.01; NS not significant. Data presented as the means ± SE (n = 6 per group).

Nowacka-Woszuk, Joanna et al., PloS one vol. 13,2 e0193464., Fig 4, doi:10.1371/journal.pone.0193464

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)
  • FuncS

Lab

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)

Acetylation Histone 3 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 20-500 ng per well) (duplicates +/- SD).

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Sensitivity

= 2 ng

검출 범위

2 - 100 ng

분석 소요 시간

2h 30m

분석 플랫폼

Microplate reader

style
left-column

보완 제품

Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

  • 4
  • 6
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

  • 5
  • 90
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

제품 세부 정보

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) allows the user to colorimetrically detect and quantify if histone H3 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115124 is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
style
left-column

제품 구성

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab115124-96Test", "assetComponentDetails": [ { "size":"3 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115124-CMP02" }, { "size":"1 x 10 mL", "name":"Color Developer", "number":"AB115124-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB115124-CMP03" }, { "size":"9 x 1 Unit", "name":"8-Well Sample Strips (with Frame)", "number":"AB115124-CMP01" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB115124-CMP08" }, { "size":"1 x 12 mL", "name":"Antibody Buffer", "number":"AB115124-CMP05" }, { "size":"1 x 20 µL", "name":"Standard Control (100 µg/ml)", "number":"AB115124-CMP07" }, { "size":"1 x 10 µL", "name":"Detection Antibody, 1 mg/ml", "number":"AB115124-CMP06" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab115124-48Test", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Buffer", "number":"AB115124-CMP05", "productcode":"" }, { "size":"2 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115124-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"10X Wash Buffer", "number":"AB115124-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Color Developer", "number":"AB115124-CMP04", "productcode":"" }, { "size":"4 x 1 Unit", "name":"8-Well Sample Strips (with Frame)", "number":"AB115124-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"Stop Solution", "number":"AB115124-CMP08", "productcode":"" }, { "size":"1 x 5 µL", "name":"Detection Antibody, 1 mg/ml", "number":"AB115124-CMP06", "productcode":"" }, { "size":"1 x 10 µL", "name":"Standard Control (100 µg/ml)", "number":"AB115124-CMP07", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
Multi
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

style
left-column

제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 24: PubMed36834533

2023

Orthodontic Compression Enhances Macrophage M2 Polarization via Histone H3 Hyperacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Wang,Sabine Groeger,Jiawen Yong,Sabine Ruf

Genes 13: PubMed36553567

2022

Pantothenate and L-Carnitine Supplementation Improves Pathological Alterations in Cellular Models of KAT6A Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Munuera-Cabeza,Mónica Álvarez-Córdoba,Juan M Suárez-Rivero,Suleva Povea-Cabello,Irene Villalón-García,Marta Talaverón-Rey,Alejandra Suárez-Carrillo,Diana Reche-López,Paula Cilleros-Holgado,Rocío Piñero-Pérez,José A Sánchez-Alcázar

Cells 11: PubMed35269485

2022

Insights into the Histone Acetylation-Mediated Regulation of the Transcription Factor Genes That Control the Embryogenic Transition in the Somatic Cells of Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Morończyk,Agnieszka Brąszewska,Barbara Wójcikowska,Karolina Chwiałkowska,Katarzyna Nowak,Anna M Wójcik,Mirosław Kwaśniewski,Małgorzata D Gaj

International journal of molecular sciences 21: PubMed32629979

2020

Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Lavinia Casati,Francesca Pagani,Roberto Maggi,Francesco Ferrucci,Valeria Sibilia

Melanoma research 28:521-526 PubMed30192303

2018

The BET-bromodomain inhibitor JQ1 mitigates vemurafenib drug resistance in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Zhao,Xiankui Cheng,Xiyuan Zhou

PloS one 13:e0193464 PubMed29474484

2018

Transgenerational effects of prenatal restricted diet on gene expression and histone modifications in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Nowacka-Woszuk,Izabela Szczerbal,Anna M Malinowska,Agata Chmurzynska

American journal of physiology. Lung cellular and 310:L700-10 PubMed26747785

2016

miR-185 mediates lung epithelial cell death after oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Duo Zhang,Heedoo Lee,Yong Cao,Charles S Dela Cruz,Yang Jin

PloS one 9:e107659 PubMed25222274

2014

Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows.

Applications

FuncS

Species

Cow

Guozhong Dong,Min Qiu,Changjin Ao,Jun Zhou, Khas-Erdene,Xi Wang,Zhu Zhang,You Yang

Renal failure 36:1078-82 PubMed24845033

2014

Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease.

Applications

FuncS

Species

Human

Gordana Kocic,Jovana Cukuranovic,Tatjana Jevtovic Stoimenov,Rade Cukuranovic,Vidosava Djordjevic,Dragan Bogdanovic,Vladisav Stefanovic
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com