- FuncS
Supplier Data
Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)
Cytokine Activation of Human Monocytic THP-1 Adhesion to HUVEC. HUVEC monolayer (left) or THP-1 cells (right) were treated with 50 ng/mL TNF-alpha or 1 µM PMA for 12 hrs. Tracker labeled THP-1 cells (50,000 cells/well) were allowed to attach to HUVEC monolayer for 1 hr. Adherent cells were lysed and quantified by as described in the Assay Protocol.
This figure demonstrates typical results with ab325586 Leukocyte-endothelium Adhesion Assay. One should use the data below for reference only. This data should not be used to interpret actual results.
- FuncS
Supplier Data
Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)
Quantitation of Human Monocytic THP-1. Tracker labeled THP-1 cells were titrated in 1X PBS, then subsequently lysed with 2X Lysis Buffer (75 µL of cell suspension was mixed with 75 µL of 2X Lysis Buffer). Fluorescence was quantified as described in Assay Protocol.
This figure demonstrates typical results with ab325586 Leukocyte-endothelium Adhesion Assay. One should use the data below for reference only. This data should not be used to interpret actual results.
- FuncS
Supplier Data
Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)
Human Monocytic THP-1 Adhesion to HUVEC Monolayer. HUVEC monolayer in 48-well plate was treated with 1 µM PMA for 12 hrs. Tracker labeled THP-1 cells were allowed to attach to HUVEC monolayer for 1 hr. Adherent cells were lysed and quantified by as described in the Assay Protocol.
This figure demonstrates typical results with ab325586 Leukocyte-endothelium Adhesion Assay. One should use the data below for reference only. This data should not be used to interpret actual results.
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Leukocyte extravasation into perivascular tissue plays a key role in inflammatory diseases. This recruitment requires leukocyte interaction with vascular endothelium and consists of multiple,consecutive processes including the capture of circulating leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration. This multistep paradigm is realized by sequential activation-dependent interactions between endothelial cell adhesion molecules and their specific ligands on leukocytes. The first step of transient adhesion and rolling is known to be mediated by an interaction of leukocyte or endothelial cellselectins and their oligosaccharide-bearing ligands. Arrest and firm adhesion of leukocytes to endothelium is dependent on the activation of β2 integrins like Mac-1 or LFA-1 on the leukocyte cell surface, followed by interaction with endothelial cell proteins belonging to the Ig superfamily such as ICAM-1.
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