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AB284573

Lysyl Oxidase Activity Assay Kit (Fluorometric)

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Lysyl Oxidase Activity Fluorometric Assay Kit (ab284573) (K928) allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media.
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Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (AB284573)
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Supplier Data

Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (AB284573)

Activity curve of abactivity curve.

Representative activity curve for cell media sample and cell media spike standard at 37°C, where cell media is test sample, cell media and inhibitor is negative control, and 150 pmol spike standard is sample and spike standard.

Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (AB284573)
  • FuncS

Supplier Data

Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (AB284573)

Enzyme activity at varying concentrations of recombinant Lysyl Oxidase.

주요 정보

검출 방식

Fluorescent

샘플 타입

Purified protein, Cell culture media

분석 유형

Enzyme activity

결과 유형

Quantitative

Sensitivity

>= 40 ng

분석 플랫폼

Microplate

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보완 제품

Assay Kits

AB112139

Lysyl Oxidase Activity Assay Kit (Fluorometric)

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Primary Antibodies

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Anti-LOX antibody [EPR4025]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (AB174316)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Lysyl Oxidase Activity Fluorometric Assay Kit (ab284573) (K928) allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media. Enzyme activity is detected upon oxidation of substrate, which results in release of reaction intermediates. Reaction intermediates are subsequently detected by LOX Probe, resulting in fluorescent signal that can be measured at Ex/Em= 535/587 nm.

Each kit includes a positive control as well as a specific lysyl oxidase inhibitor that allows the user to correct each sample for any non-specific signal. Lysyl Oxidase Activity Fluorometric Assay Kit can detect as little as 40 ng of recombinant enzyme in vitro.

This product is manufactured by BioVision, an Abcam company and was previously called K928 Lysyl Oxidase Activity Assay Kit (Fluorometric). K928-100 is the same size as the 100 test size of ab284573.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284573-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB284573-CMP02", "productcode":"AB309437" }, { "size":"1 x 45 mL", "name":"Lysyl Oxidase Assay Buffer", "number":"AB284573-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lysyl Oxidase Inhibitor", "number":"AB284573-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"H2O2 Standard", "number":"AB284573-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Lysyl Oxidase Substrate", "number":"AB284573-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lysyl Oxidase Positive Control", "number":"AB284573-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB284573-CMP06", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed : 26838787). Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (By similarity).
See full target information Protein-lysine 6-oxidase

대체 명칭 보기

Protein-lysine 6-oxidase, Lysyl oxidase, LOX
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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