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AB139457

MMP10 Inhibitor Screening Assay Kit (Fluorometric)

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(2 제품이 사용된 논문 )

Abcam MMP10 Inhibitor Screening Assay Kit (Fluorometric) (ab139457) is a complete assay system designed to screen MMP10 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl].
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Functional Studies - MMP10 Inhibitor Screening Assay Kit (Fluorometric) (AB139457)
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Supplier Data

Functional Studies - MMP10 Inhibitor Screening Assay Kit (Fluorometric) (AB139457)

Inhibition of MMP10 by NNGH

주요 정보

검출 방식

Fluorescent

샘플 타입

Inhibitor compounds

분석 유형

Enzyme activity

분석 플랫폼

Microplate reader

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam MMP10 Inhibitor Screening Assay Kit (Fluorometric) (ab139457) is a complete assay system designed to screen MMP10 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl]. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format.

This kit is useful to screen inhibitors of MMP10, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139457-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"MMP Inhibitor", "number":"AB139457-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"MMP Fluorogenic Substrate", "number":"AB139457-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Fluorometric Assay Buffer", "number":"AB139457-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-well White Microplate 1/2 Volume", "number":"AB139457-CMP06", "productcode":"" }, { "size":"1 x 30 µL", "name":"MMP10 Enzyme (Human, Recombinant)", "number":"AB139457-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Calibration Standard", "number":"AB139457-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MMP10 also known as stromelysin-2 is a member of the matrix metalloproteinase (MMP) family. This protein is involved in the degradation of components of the extracellular matrix. MMP10 weighs around 54 kDa in its native form. It gets expressed in various tissues including the skin lung and reproductive organs and plays a part in processes like tissue remodeling and repair. Unlike other MMPs MMP10 does not usually cleave collagen but breaks down proteins like elastin and gelatin. The Luminex MMP assay is a method used to study this protein's expression and regulation.
Biological function summary

MMP10 impacts the restructuring and turnover of the extracellular matrix in both normal and disease conditions. It does not work alone; it operates synergistically with other MMPs in a complex matrix framework. Within tissues MMP10 modifies the integrity of the extracellular environment affecting cell behaviors such as migration and invasion. It steps in during processes like wound healing and inflammation exemplifying its contribution to cellular repair mechanisms and their regulation.

Pathways

The activity of MMP10 intersects with the MAPK and TGF-beta signaling pathways. MMP10 facilitates these pathways by digesting extracellular matrix barriers which then influences cellular responses. MMP10's function is also linked with other metalloproteinases like MMP1 and MMP3 in the pathways. Its regulation and activation play an important role in maintaining cellular homeostasis and responding to environmental cues.

MMP10 is associated with conditions such as cancer and arthritis. In cancer its role in promoting extracellular matrix breakdown facilitates tumor cell invasion and metastasis. In arthritis MMP10 contributes to joint tissue destruction perpetuating the inflammatory cycle. This protein frequently collaborates with MMP13 in these diseases reflecting its significant part in disease progression and pathology.
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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of enzyme inhibition and medicinal chemistry 39:2423174 PubMed39513468

2024

Structure optimization and molecular dynamics studies of new tumor-selective -triazines targeting DNA and MMP-10/13 for halting colorectal and secondary liver cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Christine A Morcos,Nesreen S Haiba,Rafik W Bassily,Marwa M Abu-Serie,Amira F El-Yazbi,Omar A Soliman,Sherine N Khattab,Mohamed Teleb

Journal of enzyme inhibition and medicinal chemistry 39:2388209 PubMed39140776

2024

Non-small cell lung cancer sensitisation to platinum chemotherapy via new thiazole-triazole hybrids acting as dual T-type CCB/MMP-9 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hassan Gamal,Khadiga A Ismail,A-Mohsen M E Omar,Mohamed Teleb,Marwa M Abu-Serie,Sun Huang,Abdalla S Abdelsattar,Gerald W Zamponi,Hesham Fahmy
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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