Mouse Exosome Detection Kit

• Flow Cyt

Supplier Data

Flow Cytometry - Mouse Exosome Detection Kit (AB326320)

Dynamic range of the assay analyzed by flow cytometry. Relationship between background noise and specific signal at different exosome concentrations.

Limit of Detection (LOD) is the lowest quantity of exosomes that is distinguished from the absence of analyte (a blank value), and as reference, was determined in >0,25µg which corresponds with >1.75 * 10^8 vesicles. Whilst the upper limit or saturation level was established in 24µg. For both technical specifications were used exosome form B16F10 cell culture media (7 * 10^8 particles /µl).

Several measurements of multiple concentrations of lyophilized exosomes were analyzed across the reportable range of the kit, range the linearity of the kit in a broad range of concentrations, allowing fluorescence interpolation in the estimation of concentrations.

• Flow Cyt

Supplier Data

Flow Cytometry - Mouse Exosome Detection Kit (AB326320)

Flow analysis of exosomes bound to ab326320. Cell culture exosomes, pre-enriched using total exosome isolation from PC3 cell culture media (A), were resuspended in PBS and bound to CD63-capture beads during an overnight incubation. The following day the bead-bound exosomes were indirect stained with primary detection antibody (CD9 Biotin/CD81 Biotin) and Streptavidin-PE and analyzed by flow cytometry.