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AB289834

Mycoplasma PCR Detection Kit

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(13 제품이 사용된 논문 )

Mycoplasma PCR Detection Kit ab289834 helps to minimize false positives while ensuring coverage of over 200 species/strains of mycoplasmas with a quick protocol for routine screening.

- Results in under 2 hrs
- No DNA extraction required; add cell culture media sample directly to PCR reaction
- detect 200+ Mycoplasma species
- Includes ready-to-use primer mix and positive control
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Electrophoresis - Mycoplasma PCR Detection Kit (AB289834)
  • Electrophoresis

Lab

Electrophoresis - Mycoplasma PCR Detection Kit (AB289834)

Mycoplasma PCR Detection Kit ab289834 offers rapid and reliable screening for mycoplasma contamination in cell cultures. This gel image shows the assay results, where the positive control (+) confirms successful amplification with a 500 bp band, and samples 2-4 test positive for Mycoplasma with bands in the expected 370-550 bp range; samples 1 and 5 show no contamination. The no-template control (NTC) is clean, validating the specificity of the assay - no DNA isolation required.

주요 정보

샘플 타입

Cell culture media

결과 유형

Qualitative

제품 세부 정보

Mycoplasma PCR Detection Kit ab289834 offers rapid and reliable screening for mycoplasma contamination in cell cultures. It gives highly specific and sensitive detection of 200+ strains of Mycoplasma in less than 2 hours with a quick protocol for routine screening. In addition to the short detection process (less than 2 hours), the easy handling and high sensitivity makes this kit a convenient tool for routine examination of cell cultures and media.

Mycoplasma PCR Detection Kit protocol summary
- Prepare the PCR reagents and premix solution
- Add the sample to the PCR reaction mix.
- Perform the PCR cycling

Analyze the PCR products using agarose gel electrophoresis. A band at ~500bp indicates mycoplasma contamination.

제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Mycoplasma are a group of bacteria without a cell wall which makes them unique and adaptable to various environments. Known for their small size they have a low genomic mass. They parasitize the cells of a host organism relying on host cells for nutrients and energy. Mycoplasma can express themselves in human animal and plant hosts often found in cultures used in research laboratories. Due to their lack of cell walls they can take on various shapes which complicates their detection and monitoring in lab settings.
Biological function summary

The infection caused by these bacteria can disrupt normal cellular processes of the host. Their evasion of the immune system occurs through frequent genetic changes leading to persistent infections. Mycoplasma are not part of a complex; however they interact closely with cellular structures of the host leading researchers to employ techniques like 'mycoplasma detection kit' or 'mycoplasma PCR test' to identify contamination. These methods are necessary due to the bacteria's ability to impact cellular morphology and function.

Pathways

Various processes are disrupted upon infection by these bacteria affecting essential pathways such as DNA replication and signal transduction. For example they interfere with host cell functions through interactions with proteins like toll-like receptors which are part of the host's immune response. Mycoplasma bacteria may directly alter the metabolic flow within the pathways influencing cellular productivity and viability. The 'mycoplasma testing kit' becomes valuable in identifying these bacteria ensuring accurate experimental outcomes.

Mycoplasma bacteria are associated with respiratory infections and urogenital tract disorders. In humans Mycoplasma pneumoniae causes atypical pneumonia while Mycoplasma genitalium is linked to urogenital tract infections. Their presence in cell cultures necessitates the use of tools such as the 'mycoplasma PCR kit' to prevent compromised research. These bacteria are connected to disease processes through interactions with proteins involved in the host immune response posing challenges in treatment and necessitating continued scientific investigation.

제품 프로토콜

타겟 정보

제품이 사용된 논문 (13)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:5547 PubMed40593553

2025

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Cytosolic bacterial pathogens activate TLR pathways in tumors that synergistically enhance STING agonist cancer therapies.

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Meggie Danielson,Christopher J Nicolai,Thaomy T Vo,Natalie K Wolf,Thomas P Burke

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British journal of cancer 131:589-600 PubMed38886556

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Sutapa Sinha,Ray Guo,Miles D Del Busso,Weiguo Han,Justin Boysen,Linda E Wellik,Asish K Ghosh,Neil E Kay

Epigenetics & chromatin 17:3 PubMed38336688

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A low-input high resolution sequential chromatin immunoprecipitation method captures genome-wide dynamics of bivalent chromatin.

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Janith A Seneviratne,William W H Ho,Eleanor Glancy,Melanie A Eckersley-Maslin

Toxics 11: PubMed38133399

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Perturbations in Osteogenic Cell Fate Following Exposure to Constituents Present in Tobacco: A Combinatorial Study.

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Joseph V Madrid,Madeline K M Vera-Colón,Nicole I Zur Nieden

Oncogenesis 12:53 PubMed37949862

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Coordinate transcriptional regulation of ErbB2/3 by C-terminal binding protein 2 signals sensitivity to ErbB2 inhibition in pancreatic adenocarcinoma.

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Kranthi Kumar Chougoni,Haemin Park,Priyadarshan K Damle,Travis Mason,Bo Cheng,Martin M Dcona,Barbara Szomju,Mikhail G Dozmorov,Michael O Idowu,Steven R Grossman

Cancers 15: PubMed37835514

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Taxifolin Inhibits the Growth of Non-Small-Cell Lung Cancer via Downregulating Genes Displaying Novel and Robust Associations with Immune Evasion Factors.

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Xiaozeng Lin,Ying Dong,Yan Gu,Fengxiang Wei,Jingyi Peng,Yingying Su,Yanjun Wang,Chengzhi Yang,Sandra Vega Neira,Anil Kapoor,Damu Tang
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