JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238540

N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (ab238540) is designed for the rapid detection and quantitation of CEL protein adducts.
1 이미지
Competitive ELISA - N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (AB238540)
  • cELISA

Supplier Data

Competitive ELISA - N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (AB238540)

Example CEL-BSA ELISA Standard Curve.

Typical CEL ELISA results.

This data is for reference only and should not be used to interpret actual results.

주요 정보

검출 방식

Colorimetric

샘플 타입

Purified protein, Plasma, Serum, Cell Lysate

분석 유형

Competitive

검출 범위

0.1 - 25 µg/mL

분석 플랫폼

Microplate reader

style
left-column

보완 제품

Assay Kits

AB238543

Methylglyoxal ELISA Kit

Competitive ELISA - Methylglyoxal ELISA Kit (AB238543)

  • 0
  • 0
Primary Antibodies

AB184451

Anti-Dopamine Transporter antibody [EPR19695]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (AB184451)

  • 0
  • 0
Assay Kits

AB65347

L-Amino Acid Assay Kit

Functional Studies - L-Amino Acid Assay Kit (AB65347)

  • 0
  • 0
Primary Antibodies

AB125145

Anti-Carboxymethyl Lysine antibody [CML26]

Flow Cytometry - Anti-Carboxymethyl Lysine antibody [CML26] (AB125145)

  • 0
  • 0
style
left-column

제품 세부 정보

N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (ab238540) is designed for the rapid detection and quantitation of CEL protein adducts.

First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the pre-determined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in this kit will not cross react with CML protein adducts.

style
left-column

제품 구성

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab238540-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Protein Binding Strip Well Plate", "number":"AB238540-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238540-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Assay Diluent", "number":"AB238540-CMP05", "productcode":"" }, { "size":"1 x 300 µL", "name":"100X Conjugate Diluent", "number":"AB238540-CMP01", "productcode":"" }, { "size":"1 x 100 mL", "name":"10X Wash Buffer", "number":"AB238540-CMP02", "productcode":"" }, { "size":"1 x 30 µL", "name":"CEL-BSA Standard", "number":"AB238540-CMP07", "productcode":"" }, { "size":"1 x 20 µL", "name":"CEL Conjugate", "number":"AB238540-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Substrate Solution", "number":"AB238540-CMP10", "productcode":"" }, { "size":"1 x 20 µL", "name":"Secondary Antibody, HRP Conjugate (1000X)", "number":"AB238540-CMP08", "productcode":"" }, { "size":"1 x 10 µL", "name":"Anti-CEL Antibody (1000X)", "number":"AB238540-CMP04", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
Multi
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

N-epsilon-(Carboxyethyl) Lysine (CEL) also known as carboxyethyl lysine stands as a specific type of advanced glycation end product (AGE). It forms through the non-enzymatic glycation of proteins particularly lysine residues. CEL exhibits a variable mass depending on its attached protein matrix. Researchers identify its presence significantly in long-lived proteins such as collagen and in dietary products like dairy and roasted meats. CEL forms through Maillard reaction pathways creating cross-links in proteins which affect their structure and function.
Biological function summary

The modification of proteins by CEL impacts cellular processes. CEL alters the function and lifespan of proteins disrupting homeostasis and contributing to altered cell signaling pathways. It is associated with the alteration of extracellular matrix proteins and redox-sensitive cytoplasmic proteins. CEL is not typically part of a specific complex but its presence can influence the assembly and function of protein complexes within cells. Its influence on protein modification underlies the aging process and other cellular dysfunctions.

Pathways

CEL plays a role in oxidative stress and inflammatory pathways. It associates with the receptor for AGE (RAGE) initiating signaling cascades that contribute to inflammation and oxidative stress. The AGE-RAGE axis represents a fundamental pathway where CEL engages in modifying cellular responses and protein expressions. Other related proteins include glyoxalase enzymes that detoxify reactive carbonyl species thereby potentially counteracting CEL formation and mitigating downstream effects.

CEL contributes to the pathogenesis of diabetes and atherosclerosis among others. In diabetes CEL accumulates in vascular tissues affecting hemodynamic function and promoting complications via RAGE-related pathways. In atherosclerosis CEL-modified proteins in arterial walls favor progression due to heightened inflammation and endothelial cell dysfunction. This interaction with RAGE and other AGE-related proteins assists in understanding CEL's role in disease progression and highlights potential therapeutic targets for managing these chronic conditions.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

See full target information N(6)-(1-carboxyethyl)-L-lysine
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com