NIR Mitochondrial Membrane Potential Assay Kit (Microplate)
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Functional Studies - NIR Mitochondrial Membrane Potential Assay Kit (Microplate) (AB112150)
The decrease in NIR fluorescence with the addition of FCCP in HeLa cells.
HeLa cells were dye loaded with NIR alone, or in the presence of 20 µM FCCP for 15 minutes. The fluorescence intensity of NIR was measured 30 minutes after adding Assay buffer B (Component C) with a microplate reader at Ex/Em = 640/680 nm (cut off 665 nm).
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제품 세부 정보
ab112150 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
ab112150 NIR Mitochondria Membrane Potential Assay Kit provides all the essential components with an optimized assay method. This fluorometric assay uses our proprietary cationic MitoNIR Dye for the detection of the mitochondrial membrane potential change in cells. In normal cells, the red fluorescence intensity is increased when NIR Dye is accumulated in the mitochondria. However, in apoptotic cells, NIR stain intensity is decreased following the collapse of MMP. Cells stained with NIR Dye can be monitored fluorimetrically at Em = 660-680 nm with excitation at Ex = 620-640 nm.
ab112150 can be used for screening apoptosis activators and inhibitors. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.
Related assays
Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.
Review the metabolism assay guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The mitochondrial membrane potential drives ATP synthesis by powering ATP synthase an enzyme complex embedded in the mitochondrial membrane. This potential also plays a vital role in other processes such as calcium homeostasis and regulation of mitochondrial biogenesis. The mitochondrial membrane itself forms part of the larger mitochondrial respiratory chain complex coordinating with components like complex I (NADH: ubiquinone oxidoreductase) and complex II (succinate dehydrogenase) to maintain cell energy needs and respond to metabolic demands.
Pathways
The mitochondrial membrane potential is integral to cellular energy metabolism pathways such as the Krebs cycle and oxidative phosphorylation. Mitochondrial membrane potential modulation can affect signaling proteins like cytochrome c which is instrumental in apoptosis. Apoptotic signaling pathways involving proteins such as Bax and Bcl-2 influence the mitochondrial membrane potential and regulate cell survival or death in response to cellular stress or damage.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com