Nitroreductase Assay Kit (Luminometric)

제품 세부 정보

Nitroreductase Assay Kit (Luminometric) (ab324120) provides a highly sensitive and convenient method to quantify nitroreductase activity.

Nitroreductase assay principle
The nitroreductase assay uses a luciferin derivative that can be selectively reduced by nitroreductase. The amount of generated luciferin is then detected using a luciferase assay system.The luminescent intensity generated is proportional to the nitroreductase activity.

The assay can detect as low as 20 ng/mL of nitroreductase.

Nitroreductase assay protocol summary
- add samples and standard to wells- add first working solution and incubate for 60-min at 37°C- add second working solution and monitor luminescence in plate reader

Other Notes
Nitroreductases are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds. They are absent in mammalian cells but widespread in bacteria. Nitroreductases play a crucial role in the reduction of nitroaromatic compounds via NAD(P)H-dependent reactions. Nitroreductases are also targets for developing novel antibiotics, degradation of pollutants, and cancer therapy.