Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)

제품 세부 정보

How the assay works

In Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) protocol, tissue carbohydrates are oxidized by periodic acid forming aldehydes capable of binding with Schiff’s Solution. Visualization of Schiff’s is caused by a restoration of the dye’s quinoid structure resulting in characteristic magenta staining. Light green provides a contrasting counterstain. The staining pattern of the lymphocytes are helpful in making therapeutic decisions in established cases of lymphocytic leukemia.

Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) protocol summary:

- deparaffinize sections (if necessary) and hydrate
- immerse slide in PAS solution for 5-10 min
- rinse 4 times with water
- immerse slide in Schiff's solution for 15-30 min and rinse
- stain slide with Hematoxylin for 2-3 min and rinse
- incubate in Bluing reagent for 30 s and rinse
- incubate in Light Green Solution for 2 min and rinse
- dehydrate with alcohol, clear and mount

Staining Interpretation

Fungal Organisms Magenta
PAS Positive Material Magenta
Nuclei Black/Blue
Other Tissue Components Green/Blue
Control Tissue: Kidney, Intestine, Liver.

For the demonstration of fungal organisms in tissue sections, we recommend PAS Stain Kit (Fungus, Periodic Acid Schiff Stain) ab245886.

How other researchers are using

Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) has been used in a variety of sample type including:
- Mouse Liver tissues 1
- Mouse kidney tissues 2
- Human cornea 3

References:
1 - Liu Z et al. 2023; 2 - Meng Q et al. 2023; 3 - Kim H et al. 2023.

Related and recommended products

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Other Notes

Please note, the 125 mL selling size, was previously described as 100 Test. There has been no change to the physical product.