Peroxide Assay Kit

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Peroxide Assay Kit (ab272537) is a simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. This assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe³⁺-xylenol orange reaction, in which a purple complex is formed when Fe²⁺ provided in the reagent is oxidized to Fe³⁺ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 uM (7 ng/mL) to 30 uM (1,020 ng/mL) H₂O₂ in 96-well plate assay.

Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.