- Ex/Em 540/570 nm
- Validated as macrophage phagocytosis assay.
- includes labeled E.coli and assay buffer. Also includes quenching solution for readout on microscope / plate reader.
- FuncS
Supplier Data
Functional Studies - Phagocytosis Assay Kit (Red E. coli) (AB235901)
Inhibition of phagocytosis. J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 hour at 37°C prior to addition of 5 μL of E. coli particles. Phagocytosis was conducted for 2 hours and the amount of engulfed E. coli was determined as described in the Assay Protocol. Panel A and B : images of nontreated cells. Panel C and D : treatment with Cytochalasin D.
- Flow Cyt
Supplier Data
Flow Cytometry - Phagocytosis Assay Kit (Red E. coli) (AB235901)
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 hour at 37°C prior to addition of 5 μL of E. coli particles. Phagocytosis was conducted for 2 hours and the amount of engulfed E. coli was determined as described in the Assay Protocol. Blue line : untreated control cells; red line : macrophages with engulfed E. coli particles; green line : inhibition of phagocytosis by Cytochalasin D.
- FuncS
Supplier Data
Functional Studies - Phagocytosis Assay Kit (Red E. coli) (AB235901)
E. coli Standard curve.
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Phagocytosis Assay Kit (Red E. coli) (ab235901) uses heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptor (TLR) ligands.
Phagocytosis assay protocol summary
- add Red E. coli reagent to cells, and incubate for 2-3 hours
- harvest cells by centrifugation, remove supernatant, and resuspend cells in Assay Buffer; repeat wash step 3 times
- for fluorescent microscope or fluorometric plate reader analysis, use Quenching Solution
- analyze cells with flow cytometer, fluorescent microscope, or fluorometric plate reader
Related phagocytosis assays
Phagocytosis Assay Kit (Green E.coli) ab235900
Phagocytosis Assay Kit (Green Zymosan) ab234053
Phagocytosis Assay Kit (Red Zymosan) ab234054
Phagocytosis Assay Kit (Zymosan, Colorimetric) ab211156
Other notes
This product was previously called K964 Biovision EZCell™ Phagocytosis Assay Kit (Red E. coli). Biovision was acquired by Abcam in 2021.
Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as a pathogen in phagocytosis assays.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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제품이 사용된 논문 (1)
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STAR protocols 5:102827 PubMed38219151
2024
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