JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB273288

Phosphatidylglycerol Assay Kit (Fluorometric)

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Phosphatidylglycerol Assay Kit (Fluorometric) (ab273288) uses specific enzymes to generate an intermediate that reacts with a probe.
4 이미지
Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)
  • FuncS

Supplier Data

Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)

PG standard curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)
  • FuncS

Supplier Data

Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)

Specificity of assay, when used as described, for Phosphatidylglycerol (PG) versus Phosphatidylethanolamine (PE), Phosphatidic Acid (PA), Phosphatidylinositol (PI), and cardiolipin (CL). The amount of phospholipid for each sample was 1 nmole.

Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)
  • FuncS

Supplier Data

Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)

Determination of Phosphatidylglycerol in lipid extracted from lung tissue

Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)
  • FuncS

Supplier Data

Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)

Determination of total PG concentration in lipid extracted from brain lysate

주요 정보

검출 방식

Fluorescent

샘플 타입

Cell culture extracts, Tissue Extracts

결과 유형

Quantitative

분석 플랫폼

Microplate

style
left-column

보완 제품

Assay Kits

AB241036

Cardiolipin Assay Kit

Functional Studies - Cardiolipin Assay Kit (AB241036)

  • 5
  • 1
Primary Antibodies

AB5103

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (AB5103)

  • 4
  • 28
Assay Kits

AB242305

Lipid Assay Kit (unsaturated fatty acids)

Functional Studies - Lipid Assay Kit (unsaturated fatty acids) (AB242305)

  • 0
  • 0
Primary Antibodies

AB310335

Anti-Neutrophil Elastase antibody [EPR28386-66]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation

Multiplex immunohistochemistry - Anti-Neutrophil Elastase antibody [EPR28386-66] (AB310335)

  • 0
  • 0
style
left-column

제품 세부 정보

Phosphatidylglycerol Assay Kit (Fluorometric) (ab273288) utilizes specific enzymes to generate an intermediate that reacts with a probe. This yields a signal that can be quantified fluorometrically and is proportional to the amount of Phosphatidylglycerol (PG) present in the sample, unaffected by the presence of phosphatidic acid, cardiolipin, or other lipids. When used as described, the assay is highly selective against other phospholipids and capable of detecting as little as 20 pmoles of phosphatidylglycerol.

This product is manufactured by BioVision, an Abcam company and was previously called K488 Phosphatidylglycerol Assay Kit (Fluorometric). K488-100 is the same size as the 100 test size of ab273288.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273288-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 1", "number":"AB273288-CMP01", "productcode":"AB239210" }, { "size":"1 x 1 Vial", "name":"Phosphatidylglycerol Standard", "number":"AB273288-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PG Converter Mix", "number":"AB273288-CMP02", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB273288-CMP04", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Enzyme Mix VI", "number":"AB273288-CMP03", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphatidylglycerol (PG) also known as phosphatidyl glyceryl is a phospholipid with a molecular mass around 700-750 Da. It is an important component of cell membranes found mainly in bacteria plant chloroplasts and the lungs of mammals. In cellular membranes PG provides structural stability and fluidity playing an essential role in membrane-related processes. In the context of mammalian lungs Phosphatidylglycerol is a critical part of pulmonary surfactant which reduces surface tension in the alveoli and is vital for proper respiratory function.
Biological function summary

This lipid is a building block of membranes and acts in signaling processes. While PG does not form part of a larger protein complex it interacts closely with other lipids and proteins to maintain membrane integrity and function. The presence of Phosphatidylglycerol in pulmonary surfactant exemplifies its role in maintaining lung function and preventing alveolar collapse during exhalation. In specific bacteria PG also contributes to energy metabolism by participating in the synthesis of other phospholipids.

Pathways

Phosphatidylglycerol contributes to lipid metabolic processes and is part of the CDP-diacylglycerol pathway. This pathway is important for synthesizing other phospholipids such as cardiolipin which plays an important role in maintaining mitochondrial function. As PG interacts with proteins and enzymes related to these pathways such as phospholipase D and phosphatidylglycerophosphate synthase it influences membrane dynamics and cellular signaling.

Alterations in PG levels relate to respiratory distress syndrome (RDS) and lung diseases. A deficiency of PG in pulmonary surfactant is often observed in RDS particularly in premature infants due to inadequate surfactant production. PG also interacts with surfactant-associated proteins like SP-B and SP-C which are important for surfactant function and stability. Additionally changes in PG levels have implications for other conditions like chronic obstructive pulmonary disease (COPD) emphasizing the phospholipid's role in maintaining respiratory health.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

See full target information CID 85336641
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell metabolism 34:1960-1976.e9 PubMed36476935

2022

Lipid-laden lung mesenchymal cells foster breast cancer metastasis via metabolic reprogramming of tumor cells and natural killer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Gong,Qing Li,Jiayuan Shi,Edison T Liu,Leonard D Shultz,Guangwen Ren
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com