JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB204713

Phosphoenolpyruvic acid (PEP) Assay Kit

Be the first to review this product! 리뷰 제출

|

(5 제품이 사용된 논문 )

Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713) provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples.
2 이미지
Functional Studies - Phosphoenolpyruvic acid (PEP) Assay Kit (AB204713)
  • FuncS

Supplier Data

Functional Studies - Phosphoenolpyruvic acid (PEP) Assay Kit (AB204713)

Typical PEP Standard calibration curve using colorimetric reading.

Functional Studies - Phosphoenolpyruvic acid (PEP) Assay Kit (AB204713)
  • FuncS

Supplier Data

Functional Studies - Phosphoenolpyruvic acid (PEP) Assay Kit (AB204713)

Typical PEP Standard calibration curve using fluorometric reading.

주요 정보

검출 방식

Colorimetric/Fluorometric

샘플 타입

Tissue Lysate, Urine, Plasma, Serum, Other biological fluids, Cell Lysate

분석 유형

Enzyme activity

Sensitivity

= 1 µM

분석 플랫폼

Microplate reader

style
left-column

보완 제품

Assay Kits

AB65342

Pyruvate Assay Kit

Functional Studies - Pyruvate Assay Kit (AB65342)

  • 5
  • 2
Assay Kits

AB174097

2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric)

Functional Studies - 2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (AB174097)

  • 0
  • 0
Assay Kits

AB83396

Citrate Assay Kit

Functional Studies - Citrate Assay Kit (AB83396)

  • 0
  • 0
Assay Kits

AB65620

Glycogen Assay Kit

Functional Studies - Glycogen Assay Kit (AB65620)

  • 0
  • 0
style
left-column

제품 세부 정보

Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713) provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples.

This product is manufactured by BioVision, an Abcam company and was previously called K365 PEP Colorimetric/Fluorometric Assay Kit. K365-100 is the same size as the 100 test size of ab204713.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab204713-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB204713-CMP04", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB204713-CMP03", "productcode":"AB309440" }, { "size":"1 x 1 Vial", "name":"PEP", "number":"AB204713-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PEP Converter Mix", "number":"AB204713-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB204713-CMP01", "productcode":"AB323949" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoenolpyruvate often abbreviated as PEP is an important metabolic intermediate in glycolysis. Alternate names include phosphoenolpyruvic acid. It is a phosphorylated compound with a molar mass of 167.07 g/mol. In cells PEP is generated via the enolase enzyme converting 2-phosphoglycerate to PEP. Its high-energy phosphate group makes it critical for ATP generation in glycolysis. This molecule is expressed in the cytoplasm of all living organisms reflecting a fundamental role in cellular metabolism.
Biological function summary

The role of phosphoenolpyruvate extends beyond glycolysis. It acts as a significant player in gluconeogenesis aiding in the conversion of lactate and other substrates into glucose. PEP is an important precursor for several biosynthetic pathways including amino acid synthesis. It is not part of a molecular complex but interacts with several enzymes such as pyruvate kinase that facilitate its conversion into pyruvate releasing energy stored in its phosphate bond.

Pathways

Phosphoenolpyruvate is central to both glycolysis and gluconeogenesis. It stands at a critical juncture in the glycolytic pathway where the PEP to pyruvate conversion catalyzed by pyruvate kinase is a major step for ATP synthesis. In gluconeogenesis PEP converts back to glucose ensuring energy balance during fasting states. PEP is closely related to proteins such as pyruvate kinase and enolase which are key players in the glycolytic and gluconeogenic pathways respectively.

Alterations in phosphoenolpyruvate metabolism connect to various health conditions including cancer and diabetes. For instance dysregulated glycolysis involving PEP often occurs in cancer cells exhibiting the Warburg effect characterized by high rates of glycolysis even under oxygenated conditions. Diabetes also shows links to abnormal PEP levels affecting glucose production and metabolism. Pyruvate kinase related to PEP is another protein implicated in these disorders highlighting its role in metabolic and energy homeostasis.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

See full target information Phosphoenolpyruvate
style
left-column

제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of neuroinflammation 22:157 PubMed40517242

2025

Changes in metabolite profiles in the cerebrospinal fluid and in human neuronal cells upon tick-borne encephalitis virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Suyama,Sally Boxall,Benjamin Grace,Andrea Fořtová,Martina Pychova,Lenka Krbkova,Rupasri Mandal,David Wishart,Diane E Griffin,Daniel Růžek,Niluka Goonawardane

mBio 16:e0252424 PubMed40071948

2025

SENP1-SIRT3 axis mediates glycolytic reprogramming to suppress inflammation during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xiong,Yongliang Du,Feng Lin,Beibei Fu,Dong Guo,Zhou Sha,Rong Tian,Rui Yao,Lulu Wang,Zixuan Cong,Bohao Li,Xiaoyuan Lin,Haibo Wu

PeerJ 12:e16677 PubMed38188177

2024

Multi-omics reveals changed energy metabolism of liver and muscle by caffeine after mice swimming.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Han,Qian Jia,Yu Tian,Yan Yan,Kunlun He,Xiaojing Zhao

Journal of the European Academy of Dermatology and Venereology : JEADV 37:2067-2079 PubMed37247195

2023

Recombinant Treponema pallidum protein Tp47 promoted the phagocytosis of macrophages by activating NLRP3 inflammasome induced by PKM2-dependent glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Y-W Zheng,M Wang,J-W Xie,R Chen,X-T Wang,Y He,T-C Yang,L-L Liu,L-R Lin

Cancer & metabolism 7:8 PubMed31388420

2019

Inhibition of phosphoenolpyruvate carboxykinase blocks lactate utilization and impairs tumor growth in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emily D Montal,Kavita Bhalla,Ruby E Dewi,Christian F Ruiz,John A Haley,Ashley E Ropell,Chris Gordon,John D Haley,Geoffrey D Girnun
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com