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AB273343

Phospholipase C Activity Assay Kit (Colorimetric)

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Phospholipase C Activity Assay Kit (Colorimetric) (ab273343) is a direct assay to determine the Phospholipase C (PLC) activity in various biological samples.

대체 명칭 보기

Phospholipase C, PLC, Cereolysin A, Phosphatidylcholine cholinephosphohydrolase, plc

3 이미지
Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)
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Supplier Data

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)

Example data.

PLC Standard Curve.

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)
  • FuncS

Supplier Data

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)

Example data.

PLC activity detected in rat brain, kidney and muscle lysates.

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)
  • FuncS

Supplier Data

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)

Example data.

Reaction curve of the PLC activity.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue

분석 유형

Enzyme activity

결과 유형

Quantitative

분석 플랫폼

Microplate

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Phospholipase C Activity Assay Kit (Colorimetric) (ab273343) is a direct assay to determine the Phospholipase C (PLC) activity in various biological samples. The assay uses a specific PLC chromogenic substrate to detect PLC activity and the generated final product can be measured colorimetrically at OD 405 nm. The OD signal is proportional to the PLC activity.

The assay is rapid, sensitive and is a convenient tool for detecting PLC activity.

It can detect as low as 0.1 mU PLC activity under the assay conditions.

This product is manufactured by BioVision, an Abcam company and was previously called K2016 Phospholipase C Activity Assay Kit (Colorimetric). K2016-100 is the same size as the 100 test size of ab273343.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273343-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 61", "number":"AB273343-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PLC Substrate", "number":"AB273343-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"p-Nitrophenol Standard", "number":"AB273343-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PLC Enzyme", "number":"AB273343-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C also known as PLC is an enzyme that catalyzes the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol trisphosphate (IP3). It has several isoforms such as PLC beta and PLC gamma each with different molecular sizes. For example PLC gamma typically has a mass of about 130 kDa. This enzyme is expressed in many tissues including the brain liver and pancreas where it plays an important role in cellular signaling.
Biological function summary

The activity of Phospholipase C generates second messengers that are important for intracellular signal transduction. It functions as part of larger signaling complexes and can be involved in regulating various cellular processes like proliferation differentiation and apoptosis. This role makes it an important regulator in many physiological responses. The enzyme often interacts with membrane-bound receptors linking extracellular signals to internal cellular responses.

Pathways

Phospholipase C is an important player in the phosphatidylinositol signaling pathway and is interconnected with the calcium signaling pathway. Within these pathways PLC activity triggers the release of calcium from internal stores which in turn can activate calcium-dependent proteins such as protein kinase C. These interactions aid in modulating cellular responses to external stimuli.

PLC abnormalities have been linked to conditions like cancer and bipolar disorder. Altered PLC function can disrupt normal cellular signaling contributing to the pathogenesis of these diseases. In cancer abnormal PLC activity is tied to uncontrolled cell growth often interacting with proteins like Ras to drive oncogenic processes. In bipolar disorder disruptions in PLC function affect neurotransmitter pathways implicating changes in proteins associated with synaptic signaling.
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제품 프로토콜

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타겟 정보

Required, with sphingomyelinase, to effect target cell lysis (hemolysis).
See full target information plc
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature immunology 24:516-530 PubMed36732424

2023

Phosphoinositide acyl chain saturation drives CD8 effector T cell signaling and function.

Applications

Unspecified application

Species

Unspecified reactive species

Joy Edwards-Hicks,Petya Apostolova,Joerg M Buescher,Hannes Maib,Michal A Stanczak,Mauro Corrado,Ramon I Klein Geltink,Maria Elena Maccari,Matteo Villa,Gustavo E Carrizo,David E Sanin,Francesc Baixauli,Beth Kelly,Jonathan D Curtis,Fabian Haessler,Annette Patterson,Cameron S Field,George Caputa,Ryan L Kyle,Melanie Soballa,Minsun Cha,Harry Paul,Jacob Martin,Katarzyna M Grzes,Lea Flachsmann,Michael Mitterer,Liang Zhao,Frances Winkler,David Ali Rafei-Shamsabadi,Frank Meiss,Bertram Bengsch,Robert Zeiser,Daniel J Puleston,David O'Sullivan,Edward J Pearce,Erika L Pearce
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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