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AB238536

Protein Carbonyl ELISA Kit

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(12 제품이 사용된 논문 )

Protein Carbonyl ELISA Kit ab238536 is designed for the quantitative measurement of protein carbonyls in plasma, serum, cell lysates or purified proteins.
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Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)
  • I-ELISA

Supplier Data

Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)

Amount of Protein Carbonyl Content for Cell Lysate and BSA Standard.

STO (MEF), HeLa and MDA-231 cells were sonicated in 25mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysates and BSA Standard from Pierce BCA Protein Assay were diluted to 10 μg/mL with 1X PBS and coated onto a 96-well Protein Binding Plate. The protein carbonyl levels were determined as described in the Protein Carbonyl ELISA Protocol.

Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)
  • I-ELISA

Supplier Data

Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)

Typical Protein Carbonyl ELISA Standard Curve.

This standard curve is for demonstration only. A standard curve must be run with each protein carbonyl elisa experiment.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysate

분석 유형

Cell-based

결과 유형

Quantitative

분석 플랫폼

Microplate reader

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제품 세부 정보

Protein Carbonyl ELISA Kit (ab238536) is designed for the quantitative measurement of protein carbonyls in plasma, serum, cell lysates or purified proteins.

With this kit protein samples are first allowed to adsorb to wells of a 96-well plate and then react with dinitrophenylhydrazine (DNPH), which allows for derivatization of the carbonyl group. There are no concentration or precipitation steps, that contribute to sample loss. The kit allows detection of as little as 10 ug/mL using a standard microplate reader. The quantity of protein carbonyls in protein sample is determined by comparing its absorbance with that of a known reduced/oxidized BSA standard curve.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
Multi
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Protein carbonyls also known as carbonylated proteins serve as key markers for oxidative stress. They possess a carbonyl group introduced through oxidative modification of susceptible amino acid residues like lysine threonine proline and arginine. Protein carbonyls have no specific mass due to their varied formation but are widely detected across many cellular proteins. Carbonylation prominently expresses itself in intracellular and extracellular proteins affecting many tissues and organs. Laboratories often use the DNPH (dinitrophenylhydrazine) assay and other protein carbonylation assays to detect and quantify these modifications.
Biological function summary

Protein carbonylation alters protein function and structure. This process disrupts protein-protein interactions and impairs enzyme activity leading to protein dysfunction. Carbonylated proteins fail to perform effectively within cellular systems and accumulate in cells. These accumulations can form large aggregates which cells often recognize as damaged. Protein degradation systems struggle to efficiently degrade these aggregates which can lead to further cellular dysfunction.

Pathways

Protein carbonylation integrates into the oxidative stress response pathways. In particular it affects the proteostasis network by challenging the protein quality control systems which include chaperones and proteasomes. This can influence pathways like the ubiquitin-proteasome pathway as proteins like ubiquitin tag damaged proteins for degradation. Additionally carbonylated proteins intertwined with inflammatory pathways interact with proteins such as nuclear factor kappa B (NF-kB) contributing to pro-inflammatory signaling.

Protein carbonyls associate closely with neurodegenerative diseases like Alzheimer's disease and Parkinson's disease. The increase in oxidative stress in these conditions leads to the accumulation of carbonylated proteins which correlate with disease progression. In Alzheimer's disease oxidative damage results in the carbonylation of the amyloid precursor protein enhancing amyloid-beta accumulation. In Parkinson's disease alpha-synuclein undergoes carbonylation promoting its aggregation into Lewy bodies. These protein associations highlight the significant role of protein carbonyls in disease pathology.
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제품 프로토콜

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타겟 정보

See full target information 2-[[[(2R)-2-ethylhexyl]carbamothioylamino]methyl]-4-nitrophenolate
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제품이 사용된 논문 (12)

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