Protein Tyrosine Phosphatase Assay Kit (ab241032) enables rapid measurement of protein tyrosine phosphatase (PTPase) activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).
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Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1
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Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)
Reaction kinetics of PTPase Substrate metabolism in homogenates of human placenta (5 μg) and rat prefrontal cortex (10 μg) in the presence and absence of the PTPase inhibitor suramin.
- FuncS
Unknown
Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)
Quantification of PTPase activity in samples (mean ± SEM of 4 or more independent replicates). Assays were performed according to the kit protocol.
- FuncS
Unknown
Functional Studies - Protein Tyrosine Phosphatase Assay Kit (AB241032)
Fluorescence Standard curve. One mole of Fluorescence Standard corresponds to the metabolism of one mole of PTPase Substrate with release of one mole free inorganic phosphate.
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Protein Tyrosine Phosphatase Assay Kit (ab241032) enables rapid measurement of protein tyrosine phosphatase (PTPase) activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). A broad-spectrum PTPase inhibitor is provided for verification of specific activity in complex biological matrices, where serine/threonine phosphatases may contribute to substrate metabolism. Unlike ELISAs or malachite green-based approaches, the assay is homogeneous, continuous and does not require complicated sample processing or desalting to eliminate free phosphate. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 0.1 mU PTPase activity.
This product is manufactured by BioVision, an Abcam company and was previously called K829 Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric). K829-100 is the same size as the 100 test size of ab241032.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.
Pathways
The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.
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ACS omega 10:15511-15524 PubMed40290999
2025
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