AB108906

Tissue Factor Activity Assay Kit (Human, Colorimetric)

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Tissue Factor Activity Assay Kit (Human, Colorimetric) ab108906 is used to determine Human Tissue Factor activity in plasma, serum, urine, tissue and cell lysate samples.

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Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

FuncS

Lab

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit.

Standard curve showing concentration (pM) on X-axis and increase of O.D. per min on Y-axis (time frame : 20 min.)

FuncS

Lab

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit.

Tissue Factor measured in various biologicals showing average concentrations

FuncS

Lab

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit.

Colorimetric standard curve : mean of duplicates (+/- SD)

FuncS

Lab

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit.

Time course showing O.D.'s of one standard point and one sample (undiluted vs.diluted)

주요 정보

검출 방식

Colorimetric

샘플 타입

Urine, Plasma, Tissue, Serum, Cell Lysate

Reacts with

Human

분석 유형

Enzyme activity

결과 유형

Quantitative

Sensitivity

= 3.5 pmol/L

검출 범위

7.81 - 250 pmol/L

분석 소요 시간

분석 플랫폼

Microplate reader

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Tissue Factor Activity Assay Kit (Human, Colorimetric) ab108906 is used to determine Human Tissue Factor activity in plasma, serum, urine, tissue and cell lysate samples.

The tissue factor activity assay protocol measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.

Tissue factor activity assay protocol summary:
- add reaction mix to wells
- add standards and samples
- incubate for 30 min
- add FXa substrate
- analyze with a microplate reader every 5 min for 25 min

Other Notes
Previously called Tissue Factor Human Chromogenic Activity Assay Kit.

The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. Tissue factor binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. Tissue factor markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates.

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제품 구성

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab108906-100Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Tissue Factor Microplate (12 strips of 8 wells)", "number":"AB108906-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Sample Diluent", "number":"AB108906-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Assay Diluent", "number":"AB108906-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108906-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"FXa Substrate (Lyophilized)", "number":"AB108906-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human FVII (Lyophilized)", "number":"AB108906-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"rh Tissue Factor Standard (lipoprotein) (Lyophilized)", "number":"AB108906-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human FX (Lyophilized)", "number":"AB108906-CMP04", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

Multi

보관 정보

Please refer to protocols

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.

Biological function summary

Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.

Pathways

Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.

Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.

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제품 프로토콜

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타겟 정보

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF : VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

See full target information F3

대체 명칭 보기

CD142, Tissue factor, TF, Coagulation factor III, Thromboplastin, F3

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제품이 사용된 논문 (20)

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Product promise

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Tissue Factor Activity Assay Kit (Human, Colorimetric)

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

Functional Studies - Tissue Factor Activity Assay Kit (Human, Colorimetric) (AB108906)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (20)

Product promise